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水稻在缺氮条件下的表达谱分析

ranscription analysis of rice under nitrogen starvation

中文摘要英文摘要

本研究通过全基因组芯片对缺氮处理1h、24h、7d的水稻地上部和地下部进行基因表达谱研究,并将所得差异表达基因与拟南芥缺氮处理下的差异表达基因进行对比研究,获得双子叶和单子叶植物响应缺氮信号的保守基因。研究结果显示:总共有3518个水稻基因在缺氮条件下差异表达,它们参与多种生物学功能(比如:逆境相关、初级和次级代谢、分子运输、信号传导、转录调控、生物器官构成等);其中,有73类基因在拟南芥和水稻中同时对缺氮信号有响应。表明,在缺氮条件下,大量的基因通过改变其表达量来协调植株的各种生物学功能,以保证在逆境条件下存活并生长,并且植物体内可能存在某种共同的响应机制。

Nitrogen is an essential mineral nutrient required for plant growth and development. Insufficient nitrogen (N) supply triggers extensive physiological and biochemical changes in plants. In this study, we used Affymetrix GeneChip rice genome arrays to analyze the dynamics of rice transcriptome under N starvation. N starvation induced or suppressed transcription of 3,518 genes, representing 10.88% of the genome. These changes, mostly transient, affected various cellular metabolic pathways, including stress response, primary and secondary metabolism, molecular transport, regulatory process and organismal development. 462 or 13.1% transcripts for N starvation expressed similarly in root and shoot. Comparative analysis between rice and Arabidopsis identified 73 orthologous groups that responded to N starvation, demonstrated the existence of conserved N stress coupling mechanism among plants.

练兴明、蔡红梅、卢永恩

农作物植物学生物科学研究方法、生物科学研究技术

分子生物学水稻缺氮表达谱

nitrogenAffymetrix GeneChiptranscriptomerice

练兴明,蔡红梅,卢永恩.水稻在缺氮条件下的表达谱分析[EB/OL].(2012-03-14)[2025-07-20].http://www.paper.edu.cn/releasepaper/content/201203-437.点此复制

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