Fe3O4/Ag/纤维素酶磁性复合催化剂的制备及性质研究
Synthesis of Magnetic Fe3O4/Ag /Cellulase Composite Nanoparticles and Application in Catalytic hydrolysis of CMC-Na
利用磁性纳米Fe3O4做种子，通过甲醛还原银氨溶液，得到了粒径小于30nm的球形Fe3O4/Ag复合纳米颗粒。复合颗粒通过碳化二亚胺盐酸盐（EDC?HCl）活化之后，成功实现了对纤维素酶的固载。磁性复合催化剂在室温下展示了良好的磁响应性和超顺磁性，其饱和磁化强度（Ms）达到了24 emu/g。载酶磁性复合颗粒对羧甲基纤维素钠（CMC-Na）具有良好的酶促催化水解作用，且循环使用6次后，纤维素酶活性仍可保持36%以上。这对提高纤维素酶利用效率、降低酶促反应生产成本，开发可再生洁净能源等都具有重要的意义。
In the presence of magnetic nano-Fe3O4 seed, spherical Fe3O4/Ag composite catalysis with a size smaller than 30nm were synthesized by the reduction of silver ammonia solution with formaldehyde. Cellulase can be fabricated on the surface of Fe3O4/Ag by the activation of carbodimide hydrochloride (EDC (HCl). The composite catalysts present a good magnetic responsiveness and superparamagnetism at room temperature, and its saturation magnetization (Ms) reached 24emu/g. The composite catalysts has a good enzyme-catalyzed hydrolysis effect on sodium carboxymethyl cellulose (CMC-Na), the relate activity of cellulase can still maintain 36 percent after use 6 times. It is great significant to improve the efficiency of cellulase, reduce production costs of enzymatic reaction, develop renewable clean energy.
王秋静、沈玉华、黄建洲、李士阔
纤维素化学工业生物工程学化学工业概论
Fe3O4/Ag纤维素酶固定化酶活
Magnetic nanoparticles Fe3O4/AgellulaseImmobilizationEnzyme activity
王秋静,沈玉华,黄建洲,李士阔.Fe3O4/Ag/纤维素酶磁性复合催化剂的制备及性质研究[EB/OL].(2010-05-17)[2025-07-20].http://www.paper.edu.cn/releasepaper/content/201005-349.点此复制
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