A virus-encoded protein suppresses methylation of the viral genome through its interaction with AGO4 in the Cajal body
A virus-encoded protein suppresses methylation of the viral genome through its interaction with AGO4 in the Cajal body
ABSTRACT DNA methylation is a eukaryotic anti-viral defence mechanism. In plants, establishment of de novo DNA methylation is regulated by the RNA-directed DNA methylation pathway, which requires AGO4 function. The genome of the plant DNA viruses geminiviruses replicates in the nuclei of infected cells through not yet fully understood mechanisms and is subjected to methylation, a modification that negatively impacts infectivity. In Tomato yellow leaf curl virus, the virus-encoded V2 protein suppresses methylation of the viral DNA. Here, we identify AGO4 as a physical interactor of V2. AGO4 mediates methylation of the viral genome, which is countered by V2. Accordingly, virulence of a V2 mutant virus is partially restored by AGO4 silencing, hinting at the inhibition of AGO4 as a crucial virulence function of V2. Virus-produced V2 does not affect accumulation of viral small interfering RNA nor prevents their loading into AGO4, but impairs binding of this protein to the viral DNA. Importantly, the association between V2 and AGO4 occurs in the Cajal body, uncovering this subnuclear compartment as a crucial site in the viral cycle.
Wang Liping、Zhang Guiping、He Li、Zhu Jian-Kang、Lozano-Duran Rosa、Ding Yi
Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences Center for Excellence in Molecular Plant Science, Chinese Academy of Sciences||University of the Chinese Academy of SciencesShanghai Center for Plant Stress Biology, Chinese Academy of Sciences Center for Excellence in Molecular Plant Science, Chinese Academy of SciencesShanghai Center for Plant Stress Biology, Chinese Academy of Sciences Center for Excellence in Molecular Plant Science, Chinese Academy of SciencesShanghai Center for Plant Stress Biology, Chinese Academy of Sciences Center for Excellence in Molecular Plant Science, Chinese Academy of SciencesShanghai Center for Plant Stress Biology, Chinese Academy of Sciences Center for Excellence in Molecular Plant Science, Chinese Academy of SciencesShanghai Center for Plant Stress Biology, Chinese Academy of Sciences Center for Excellence in Molecular Plant Science, Chinese Academy of Sciences||University of the Chinese Academy of Sciences
分子生物学植物学遗传学
GeminivirusAGO4Cajal bodyDNA methylationvirus
Wang Liping,Zhang Guiping,He Li,Zhu Jian-Kang,Lozano-Duran Rosa,Ding Yi.A virus-encoded protein suppresses methylation of the viral genome through its interaction with AGO4 in the Cajal body[EB/OL].(2025-03-28)[2025-06-03].https://www.biorxiv.org/content/10.1101/811091.点此复制
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