八角茴香果实化学成分及其抗氧化活性 研究
Chemical constituents from the fruits of Illicium verum and their antioxidant activities
王一谋 陈章贤 沈晓江 朱奎霖 董维茂 尹蕊 何红平 董发武
作者信息
摘要
为探究八角茴香（ Illicium verum）果实的化学成分及其抗氧化活性，该研究 采 用硅胶、 凝胶 、 HPLC等色谱技术对八角茴香果实的化学成分进行分离 提纯 利用理化特性和波谱数据并结合参考文献 确定了所得 天然产物 的分子结构 并 采 用 DPPH法 评价化合物 抗氧化活性。 结果表明：1 从八角茴香果实中分离得到 23个化合物，分别鉴定为 1 hydroxy 1--( 2 one (1)、 ethyl 5--( 3, 4 dihydroxy 1 cyclohexene 1 carboxylate (2)、 isoshonanin (3)、 yunnanensin A (4)、 isolariciresinol 9 acetate (5)、 isolariciresinol acetate (6)、 kinsenone (7)、对羟基肉桂酸 (8)、杜荆素 (9)、芹菜素 7 O 芸香糖苷 (10)、槲皮素 (11)、 (2E, 脱落酸 (12)、 paratrimerins H (13)、 1(4 乙基苯基 1, 2 乙二醇 (14)、麦芽酚 (15)、 丁香酸 (16)、 莽草酸 (17)、莽草酸乙酯 (18)、莽草酸正丁酯 (19)、原儿茶酸 (20)、原儿茶酸甲酯 (21)、原儿茶酸乙酯 (22) 和 原儿茶酸丁酯 (23)。 其中化合物 1、 2为新天然产物，化合物 1 10、 13 15、 19、 21 23为首次从八角属植物中分离得到，化合物 12、 18为首次从 八角茴香果实 中分离得到。 2 化合物 11、 16、 21 23比阳性对照 VC展示出 更强的 DPPH自由基清除活性 ，其 IC50值 分别 为 60.15±1.72）、 35.51±0.50）、 52.25±0.73）、33.34±0.94）、 30.29±0.67 μmol· L 1。本研究结果 揭示了 八角茴香的化学成分，为 深入开展 八角茴香 抗氧化活性研究 提供 科学 依据。
Abstract
To study the chemical constituents and antioxidant activity of the fruit of Illicium verum, this study used various chromatography techniques, like silica gel, Sephadex LH 20, and HPLC, etc., were used to isolate and purify the chemical components of I. verum fruit. The structures of the isolates were identified using physicochemical constants combined with spectral data, and DPPH method was used to evaluate the antioxidant activity of the compounds. The results were as follows: (1) A total of 23 compounds were isolated and identified as 1 hydroxy 1--( methoxyphenyl)propan 2 one (1), ethyl 5 ( 3,4 dihydroxy 1 cyclohexene 1 carboxylate (2), isoshonanin (3), yunnanensin A (4), isolariciresinol 9 acetate (5), isolariciresinol acetate (6), kinsenone (7), 4 coumaric acid (8), vitexin (9), apigenin 7 O rutinoside (10), quercetin (11), (2E, abscisic acid (12), paratrimerins H (13), 1--(41,2 ethanediol (14), maltol (15), syringic acid (16), shikimic acid (17), shikimic acid ethyl ester (18), shikimic acid n butyl ester (19), protocatechuic acid (20), protocatechuic acid methyl ester (21), protocatechuic acid ethyl ester (22) and protocatechuic acid n butyl ester (23). Among with, compounds 1 and 2 were new natural products, compounds 1 10, 13 15, 19, and 21 23 were isolated from Illicium for the first time, while compounds 12 and 18 are isolated from I. verum for the first time. (2) Compounds 11, 16, and 21 23 exhibited stronger DPPH radical scavenging activity than the positive control VC, The IC50 of the five compounds were (60.151.72), (35.510.50), (52.250.73), (33.340.94), (30.290.67) molL 1 respectively. This study reveals the chemical composition of star anise, and provides a reference for indepth research on the antioxidant activity of star anise.关键词
八角茴香/新天然产物/苯丙素/化学成分/结构鉴定/DPPHKey words
Illicium verum/new natural product/phenylpropanoid/chemical constituents/structure identification/DPPH引用本文复制引用
王一谋,陈章贤,沈晓江,朱奎霖,董维茂,尹蕊,何红平,董发武.八角茴香果实化学成分及其抗氧化活性 研究[EB/OL].(2024-10-25)[2026-04-05].https://chinaxiv.org/abs/202411.00092.学科分类
生物科学研究方法、生物科学研究技术/生物化学/植物学
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