代谢工程强化Rhodococcus jostii LETBE 8896利用PET降解液合成番茄红素

Polyethylene terephthalate (PET) is a widely used plastic, but its improper disposal causes serious environmental pollution. As an innovative solution, the development of PET waste into high-value chemicals through bioconversion has received much attention. In this study, we employed strategies such as enhancement of the MEP pathway and optimisation of expression components to improve the efficiency of strain R. jostii LETBE 8896 in the synthesis of lycopene using PET degradate, which resulted in an 806-fold increase in the production of lycopene over the original strain, reaching 3226 μg/L. In addition, we also explored the ability of strain R. jostii LETBE 8896\'s substrate broad-spectrum, and attempted to produce lycopene by using four plastic degradation fluids, PBAT, PBSA, PBST, and PBT, in addition to PET, as the carbon source, which further proved that the main carbon source for lycopene production by strain R. jostii LETBE 8896 was TPA.The present study has provided a new method to provide a new way to produce lycopene from plastic wastes. This study provides a new idea for the high-value conversion of plastic waste and achieves the highest titre of lycopene synthesis from PET degradate reported to date, providing a new approach to waste management and contributing to the goals of a circular economy and global sustainable development.