高产SOD酶乳酸菌和酵母菌的筛选及优化

Superoxide dismutase (SOD) is a crucial endogenous antioxidant enzyme with significant potential in the development of antioxidant and whitening products. This study aimed to screen lactic acid bacteria and yeast strains with high SOD activity, optimize their culture conditions to enhance SOD production, and investigate the enzymatic properties of crude enzyme extracts. The findings provide new insights into developing probiotic-derived whitening products. A total of 37 lactic acid bacteria and 75 yeast strains were isolated from fermented foods and laboratory-preserved strains. Through SOD activity assays and mutagenesis screening, Levilactobacillus brevis M-6 (SOD activity: 658.71 U/g wet weight) and Saccharomyces cerevisiae SC (SOD activity: 3480.6Screening and Fermentation Optimization of High-Yield SODScreening and Fermentation Optimization of High-Yield SOD-Producing Lactic Acid Bacteria and Yeast-Producing Lactic Acid Bacteria and Yeast8 U/g wet weight) were identified as the most promising strains. Shake flask experiments were conducted to optimize the fermentation process, including medium composition, fermentation conditions, and metal ion supplementation. As a result, SOD activity was significantly improved, reaching 1273.69 U/g for L. brevis M-6 and 5042.31 U/g for S. cerevisiae SC. Further analysis demonstrated that the selected strains exhibited excellent stability and potential probiotic applications. This study innovatively applied lactic acid bacteria and yeast in high-SOD-activity microbial screening and optimization, while also exploring their stability and optimal conditions. These findings lay the foundation for future antioxidant product development.