基于细胞吸附与离子交换色谱的高纯度Nisin Z回收新策略

Nisin is a natural antimicrobial peptide widely used in the food industry owing to its effectiveness and safety. High-purity nisin has shown significant potential for medical applications, including cancer treatment, skin infections, and oral health, owing to its enhanced bioactivity and safety. However, conventional purification methods often suffer from low efficiency, which makes it challenging to achieve high purity and recovery simultaneously. This study introduced an innovative purification process specifically designed to produce high-purity nisin. This process involved nisin adsorption onto microbial cells during fermentation, with approximately 48% of the nisin bound to the cells. After fermentation, 0.02 M hydrochloric acid was used to desorb cell-bound nisin, purified via weak acid cation-exchange chromatography with D155 resin. A novel salt-free hydrochloric acid-ethanol solution was employed as the eluent, achieving over 95% elution efficiency and yielding nisin with a specific activity of 80,216.43 international units (IU)/mg protein. The freeze-dried product obtained from the ion-exchange chromatography elution represented high-purity nisin Z, with a 36,372.66 IU/mg titer. Stability tests over three batch runs confirmed the reproducibility of the process, which achieved a high-purity nisin yield of 94.42%. This streamlined process substantially enhanced the purity and yield of nisin, offering a scalable approach for industrial production in the pharmaceutical field.