拟南芥中m6A去甲基化酶ALKBH9B响应远红光的机制探究

Light is an essential non-biological environmental factor for plants, influencing nearly every stage of their growth and development. m6A modification is one of the most abundant RNA modifications in eukaryotes, regulated by methyltransferases, demethylases, and methyl readers. Existing studies have revealed a connection between m6A modification and light responses; however, the mechanisms of demethylases in light signaling pathways remain unclear. To investigate the role and mechanism of the m6A demethylase ALKBH9B in far-red light signaling in Arabidopsis thaliana , this study used the alkbh9b mutant as a model. Through phenotypic analysis, we found that the absence of ALKBH9B inhibits hypocotyl growth under far-red light. Furthermore, by integrating RNA sequencing (RNA-seq) technology, we compared differentially expressed genes between the alkbh9b mutant and wild-type under far-red light conditions and identified 1,037 candidate genes responsive to far-red light and potentially associated with ALKBH9B. These findings lay the molecular foundation for identifying downstream genes of ALKBH9B in the far-red light signaling pathway and provide new insights into the role of m6A modifications in far-red light adaptation in plants.