哈茨木霉T9131 鉴定及其拮抗病原菌和诱导黄芪抗病的作用分析

he fungus Trichoderma spp. is ubiquitously detectable in soil and plant roots, and it plays a crucial role in the biological control of agriculture. To explore the influence ofTrichoderma spp. on the resistance of Astragalus membranaceus to root rot, the aim of this studyis to isolate and identify type of Trichoderma from Astragalus membranaceus root rot, andsubsequently investigate its antagonistic confrontation on pathogen and analyze its effect onchange of resistance physiological indexes of Astragalus membranaceus. The type of Trichodermawas determined through morphological characteristics, ITS and tef1 sequence analysis. Theantagonistic confrontation of Trichoderma isolate against Fusarium solani HYFS-1 was analyzedby plate confrontation assay. To determine the role of Trichoderma isolate in inducing theresistance of Astragalus membranaceus, the physiological indexes such as catalase (CAT),superoxide dismutase (SOD), phenylalanine ammonlyase (PAL), peroxidase (POD) and proline(Pro) were determined. The results were as follows(1) The Trichoderma type from Astragalusmembranaceus root rot was identified as Trichoderma harzianum and named T9131. (2) Theinhibition rate of Trichoderma harzianum T9131 against Fusarium solani HYFS-1 reached 72% 1% after 6 days of two confrontation. (3) Compared to HYFS-1 alone, T9131 significantlyenhanced SOD activity at 0h after HYFS-1 infection. At the 24h infection, T9131 markedlyincreased SOD activity, POD activity, and Pro content. By the 48h infection, T9131 significantlyelevated POD, PAL activity, and Pro content. To sum up, Trichoderma harzianum T9131 cancontrol Astragalus membranaceus root rot by directly inhibiting the growth of HYFS-1 and couldenhance SOD, POD, PAL acitivities and Pro content of Astragalus membranaceus underFusarium solani HYFS-1 stress. This research will lay the foundation for exploring themechanism of Trichoderma harzianum against Astragalus membranaceus root rot.