籽用南瓜多糖提取、表征及抗氧化活性研究

In this paper, seed-used pumpkin seeds are primarily utilized for oil extraction or food production, while the flesh is an agricultural byproduct. In this study, a polysaccharide PPFS was extracted from seed-used pumpkin flesh using a deep eutectic solvent (DES) method. PPFS was further purified via ion exchange and gel filtration chromatography to obtain PPFS-3. Comprehensive structural characterization revealed that PPFS-3 has a molecular weight of 2.02×10? Da and is composed of nine monosaccharides: fucose, arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid,and glucuronicacid, with a molar ratio of 1.2:7.2:9.85:7.16:1.16:0.74:0.52:71.21:1.41. This study represents the first primary structural characterization of polysaccharides from seed-used pumpkin. The backbone of PPFS-3 primarily consists of galactose, galacturonic acid, and rhamnose, while arabinose predominantly exists in its branched chains, indicating that PPFS-3 is a structurally complex acidic polysaccharide. Antioxidant assays demonstrated that PPFS-3 exhibits significant dose-dependent antioxidant activity, suggesting its potential as a functional additive in food and cosmetic industries.