丹霞小花苣苔叶片离体培养与植株再生技术研究

Primulina danxiaensis, an endemic species of the Danxia landform within the Gesneriaceaefamily, exhibits a narrow distribution range and a limited population size, thereby necessitating propagationand conservation via plant tissue culture techniques. In this paper, in order to establish the tissue culture andrapid propagation technical system of P. danxiaensis, the leaf segments of P. danxiaensis were used asexplants to screen the appropriate surface disinfection time with HgCl, the culture media for adventitiousbud induction, bud proliferation and rooting, as well as the transplanting substrates for tissue-culturedseedlings. The results were as follows: (1) The optimal disinfection procedure involved a 30-second immersion in 75% alcohol, followed by a 6-minute soak in 0.1% HgCl2, achieving an 84.95% survival rateof leaf explants. (2) For bud induction, the most effective medium was found to be 1/2MS supplementedwith 6-benzyladenine (6-BA) 2 mgL1 and -naphthalene aceticacid (NAA) 0.1 mgL1, resulting in a100% bud induction rate and an average of 38.35 buds per leaf explant after 40 days of culture. (3) Budproliferation was optimally achieved on 1/2MS medium containing 6-BA 3 mgL1 and NAA 0.2 mgL1 ,yielding a proliferation coefficient of 7.54 over a 50-day period. (4) Rooting was successfully inducedusing 1/2MS medium supplemented with NAA 0.5 mgL1 , leading to a 100% rooting rate and an averageof 26.28 roots per plant after 30 days. (5) The tissue-cultured seedlings were successfully acclimatized andtransplanted into three different mixed substrates: a mixture of leaf mould from Karst landform, perlite, andvermiculite (1:1:1, V/V/V), peat soil, perlite, and vermiculite (1:1:1, V/V/V), and perlite and vermiculite (1:1,V/V), all with a 100% survival rate and demonstrating robust growth. This study is capable of achievinglarge-scale propagation of P. danxiaensis, a result that significantly contributes to both its resourceprotection and utilization.