防风地上部分化学成分及抗炎活性研究

In order to investigate the chemical components and anti-inflammatory activity fromthe aerial part of Saposhnikovia divaricata. Silica gel, ODS and HPLC etc. were utilized for theseparation and purification of the 70% ethanol extract. The structures of the isolated compoundswere characterized by integrating physicochemical properties and spectroscopic data including1H-NMR, 13C-NMR, and MS. Their inhibitory activity of the compounds on nitric oxide (NO) was determined by lipopolysaccharide(LPS)-induced RAW264.7 cell model. The results were asfollow: (1)Fifteen compounds were isolated from the 70% ethanol extract of the aerial parts ofSaposhnikovia divaricata. They were identified as gingerglycolipid A (1), (E)-2-hexenyl-O--Dglucopyranoside(2), (Z)-3-hexenyl-1-O--D-glucopyranoside (3), hexyl-1-O--Glucopyranoside(4), sachalinoside B (5), 5,6-dihydroxy-3-(-D-glucopyranosyloxy)-7-megastigmen-9-one (6),phenethyl--D- glucopyranoside (7), ethylgallate (8), vanillic acid (9), grasshopper ketone (10),2-ethoxy-2-(4-hydroxyphenyl)-ethanol (11), 2-methoxy-2-(4-hydroxyphenyl)ethanol (12), 1,2,3,4,6-penta-O-gally--D-glucopyranose (13), (-)-angelica angellinol-2-O--D-furan celeryglycosyl-(16)--D-glucopyranoside(14), (9Z,12Z)-N-(2-hydroxyethyl)octadeca-9,12-dienamide(15). Among them, compounds 1-5 and 7-10 were isolated from plants of the Umbelliferae for thefirst time, and compounds 11-15 were discovered from plants of the Saposhnikovia genus for thefirst time. (2) In vitro anti-inflammatory activity experiments were conducted on compounds 1-15.The results showed that compounds 1, 3, 4, 7, 9, 12 and14 could inhibit the release of NO fromRAW 264.7 cells induced by LPS.