Comparative genomics of human Lactobacillus crispatus isolates reveals genes for glycosylation and glycogen degradation: Implications for in vivo dominance of the vaginal microbiota
Comparative genomics of human Lactobacillus crispatus isolates reveals genes for glycosylation and glycogen degradation: Implications for in vivo dominance of the vaginal microbiota
ABSTRACT BackgroundA vaginal microbiota dominated by lactobacilli (particularly Lactobacillus crispatus) is associated with vaginal health, whereas a vaginal microbiota not dominated by lactobacilli is considered dysbiotic. Here we investigated whether L. crispatus strains isolated from the vaginal tract of women with Lactobacillus-dominated vaginal microbiota (LVM) are pheno- or genotypically distinct from L. crispatus strains isolated from vaginal samples with dysbiotic vaginal microbiota (DVM). ResultsWe studied 33 L. crispatus strains (n=16 from LVM; n=17 from DVM). Comparison of these two groups of strains showed that, although strain differences existed, both groups were heterofermentative, produced similar amounts of organic acids, inhibited Neisseria gonorrhoeae growth and did not produce biofilms. Comparative genomics analyses of 28 strains (n=12 LVM; n=16 DVM) revealed a novel, 3-fragmented glycosyltransferase gene that was more prevalent among strains isolated from DVM. Most L. crispatus strains showed growth on glycogen-supplemented growth media. Strains that showed less efficient (n=6) or no (n=1) growth on glycogen all carried N-terminal deletions (respectively, 29 and 37 amino acid-deletions) in a putative pullulanase type I gene. DiscussionL. crispatus strains isolated from LVM were not phenotypically distinct from L. crispatus strains isolated from DVM, however, the finding that the latter were more likely to carry a 3-fragmented glycosyltransferase gene may indicate a role for cell surface glycoconjugates, which may shape vaginal microbiota-host interactions. Furthermore, the observation that variation in the pullulanase type I gene associated with growth on glycogen discourages previous claims that L. crispatus cannot directly utilize glycogen.
de Vries Henry、Bruisten Sylvia M.、Tytgat Hanne L. P.、Angelino-Bart Alie de Kat、Reid Gregor、Schuren Frank、Molenaar Douwe、Swanenburg Jorne、van der Veer Charlotte、Kort Remco、Hertzberger Rosanne Y.
Public Health Service, GGD, Department of Infectious diseases||Amsterdam Public Health research instituteDepartment of Molecular Cell Biology, Faculty of Earth and Life Sciences, VU University||Amsterdam Public Health research instituteInstitute of MicrobiologyNetherlands Organization for Applied Scientific Research (TNO), Microbiology and Systems BiologyCanadian R&D Centre for Human Microbiome and Probiotics, Lawson Health Research Institute||Departments of Microbiology and Immunology, and Surgery, Western UniversityNetherlands Organization for Applied Scientific Research (TNO), Microbiology and Systems BiologyDepartment of Molecular Cell Biology, Faculty of Earth and Life Sciences, VU UniversityDepartment of Molecular Cell Biology, Faculty of Earth and Life Sciences, VU University||Netherlands Organization for Applied Scientific Research (TNO), Microbiology and Systems BiologyPublic Health Service, GGD, Department of Infectious diseasesDepartment of Molecular Cell Biology, Faculty of Earth and Life Sciences, VU University||Netherlands Organization for Applied Scientific Research (TNO), Microbiology and Systems BiologyDepartment of Molecular Cell Biology, Faculty of Earth and Life Sciences, VU University
微生物学分子生物学遗传学
de Vries Henry,Bruisten Sylvia M.,Tytgat Hanne L. P.,Angelino-Bart Alie de Kat,Reid Gregor,Schuren Frank,Molenaar Douwe,Swanenburg Jorne,van der Veer Charlotte,Kort Remco,Hertzberger Rosanne Y..Comparative genomics of human Lactobacillus crispatus isolates reveals genes for glycosylation and glycogen degradation: Implications for in vivo dominance of the vaginal microbiota[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/441972.点此复制
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