Evaluation of K18- hACE2 mice as a model of SARS-CoV-2 infection
Evaluation of K18- hACE2 mice as a model of SARS-CoV-2 infection
Abstract/Summary Murine models of SARS-CoV-2 infection are critical for elucidating the biological pathways underlying COVID-19 disease. Because human ACE2 is the receptor for SARS-CoV-2, mice expressing the human ACE2 gene have shown promise as a potential model for COVID-19. Five mice from the transgenic mouse strain K18-hACE2 were intranasally inoculated with SARS-CoV-2 Hong Kong/VM20001061/2020. Mice were followed twice daily for five days and scored for weight loss and clinical symptoms. Infected mice did not exhibit any signs of infection until day four, when weight loss, but no other obvious clinical symptoms were observed. By day five all infected mice had lost around 10% of their original body weight, but exhibited variable clinical symptoms. All infected mice showed high viral titers in the lungs as well as altered lung histology associated with proteinaceous debris in the alveolar space, interstitial inflammatory cell infiltration and alveolar septal thickening. Overall, these results show that symptomatic SARS-CoV-2 infection can be established in the K18-hACE2 transgenic background and should be a useful mouse model for COVID-19 disease.
Moreau G. Brett、Burgess Stacey L.、Petri William A. Jr.、Sturek Jeffrey M.、Mann Barbara J.、Donlan Alexandra N.
Department of Medicine, Division of Infectious Diseases, University of Virginia School of MedicineDepartment of Medicine, Division of Infectious Diseases, University of Virginia School of MedicineDepartment of Medicine, Division of Infectious Diseases, University of Virginia School of Medicine||Department of Microbiology, Immunology, & Cancer Biology, University of Virginia School of Medicine||Department of Pathology, University of Virginia School of MedicineDepartment of Medicine, Division of Pulmonary and Critical Care Medicine, University of Virginia School of MedicineDepartment of Medicine, Division of Infectious Diseases, University of Virginia School of Medicine||Department of Microbiology, Immunology, & Cancer Biology, University of Virginia School of MedicineDepartment of Microbiology, Immunology, & Cancer Biology, University of Virginia School of Medicine
医学研究方法基础医学生物科学研究方法、生物科学研究技术
Moreau G. Brett,Burgess Stacey L.,Petri William A. Jr.,Sturek Jeffrey M.,Mann Barbara J.,Donlan Alexandra N..Evaluation of K18- hACE2 mice as a model of SARS-CoV-2 infection[EB/OL].(2025-03-28)[2025-08-29].https://www.biorxiv.org/content/10.1101/2020.06.26.171033.点此复制
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