Cytokine receptor-Eb1 interaction couples cell polarity and fate during asymmetric cell division
Cytokine receptor-Eb1 interaction couples cell polarity and fate during asymmetric cell division
Abstract Asymmetric stem cell division is a critical mechanism for balancing self-renewal and differentiation. Adult stem cells often orient their mitotic spindle to place one daughter inside the niche and the other outside of it to achieve asymmetric division. It remains unknown whether and how the niche may direct division orientation. Here we discover a novel and evolutionary conserved mechanism that couples cell polarity to cell fate. We show that the cytokine receptor homolog Dome, acting downstream of the niche-derived ligand Upd, directly binds to the microtubule-binding protein Eb1 to regulate spindle orientation in Drosophila male germline stem cells (GSCs). Dome’s role in spindle orientation is entirely separable from its known function in self-renewal mediated by the JAK-STAT pathway. We propose that integration of two functions (cell polarity and fate) in a single receptor is a key mechanism to ensure an asymmetric outcome following cell division.
Sept David、Mordovanakis Aghapi、Mayer Michael、Cummings Ryan、Chen Cuie、Yamashita Yukiko M.、Hunt Alan J.
Department of Biomedical Engineering, University of MichiganDepartment of Biomedical Engineering, University of MichiganDepartment of Biomedical Engineering, University of MichiganLife Sciences Institute, Department of Cell and Developmental Biology, University of Michigan||Howard Hughes Medical Institute, University of MichiganLife Sciences Institute, Department of Cell and Developmental Biology, University of Michigan||Howard Hughes Medical Institute, University of Michigan||Department of Biomedical Engineering, University of MichiganLife Sciences Institute, Department of Cell and Developmental Biology, University of Michigan||Howard Hughes Medical Institute, University of MichiganDepartment of Biomedical Engineering, University of Michigan
细胞生物学分子生物学遗传学
Sept David,Mordovanakis Aghapi,Mayer Michael,Cummings Ryan,Chen Cuie,Yamashita Yukiko M.,Hunt Alan J..Cytokine receptor-Eb1 interaction couples cell polarity and fate during asymmetric cell division[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/229047.点此复制
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