An In Vitro Vascularized Micro-Tumor Model of Human Colorectal Cancer Recapitulates In Vivo Drug Responses
An In Vitro Vascularized Micro-Tumor Model of Human Colorectal Cancer Recapitulates In Vivo Drug Responses
Around 95% of anti-cancer drugs that show promise during preclinical study fail to gain FDA-approval for clinical use. This failure of the preclinical pipeline highlights the need for improved, physiologically-relevant in vitro models that can better serve as reliable drug-screening tools. The vascularized micro-tumor (VMT) is a novel three-dimensional model system that recapitulates the complex human tumor microenvironment, including perfused vasculature, within a transparent microfluidic device, allowing real-time study of drug responses and tumor-stromal interactions. Here we have validated the VMT platform for the study of colorectal cancer (CRC), the second leading cause of cancer-related deaths, by showing that gene expression, tumor heterogeneity, and treatment response in the VMT more closely model CRC tumor clinicopathology than current standard drug screening modalities, including 2-dimensional (2D) monolayer culture and 3-dimensional (3D) spheroids.
Chen George T.、Lawson Devon A.、Movsesyan Silva、Hatch Michaela M.、Nee Kevin、Lee Abraham P.、Hoang Tuyen、Wu Jie、Hachey Stephanie J.、Waterman Marian L.、Hughes Christopher C.W.、Burton-Sojo Giselle、Gomez Samantha、Celaya Elizabeth、Davis Ryan T.、Tankanzyan Ani、Nguyen Quy H.、Kessenbrock Kai、Zhao Da、Pervolarakis Nicholas
Department of Microbiology and Molecular Genetics, University of CaliforniaDepartment of Physiology and Biophysics, University of CaliforniaDepartment of Molecular Biology and Biochemistry, University of CaliforniaDepartment of Molecular Biology and Biochemistry, University of CaliforniaDepartment of Biological Chemistry, University of CaliforniaDepartment of Biomedical Engineering, University of CaliforniaDepartment of Biostatistics, University of CaliforniaDepartment of Biological Chemistry, University of CaliforniaDepartment of Molecular Biology and Biochemistry, University of CaliforniaDepartment of Microbiology and Molecular Genetics, University of CaliforniaDepartment of Molecular Biology and Biochemistry, University of California||Department of Biomedical Engineering, University of CaliforniaDepartment of Molecular Biology and Biochemistry, University of CaliforniaDepartment of Molecular Biology and Biochemistry, University of CaliforniaDepartment of Molecular Biology and Biochemistry, University of CaliforniaDepartment of Physiology and Biophysics, University of CaliforniaDepartment of Molecular Biology and Biochemistry, University of CaliforniaDepartment of Biological Chemistry, University of CaliforniaDepartment of Biological Chemistry, University of CaliforniaDepartment of Biomedical Engineering, University of CaliforniaCenter for Complex Biological Systems, University of California
医学研究方法肿瘤学基础医学
Chen George T.,Lawson Devon A.,Movsesyan Silva,Hatch Michaela M.,Nee Kevin,Lee Abraham P.,Hoang Tuyen,Wu Jie,Hachey Stephanie J.,Waterman Marian L.,Hughes Christopher C.W.,Burton-Sojo Giselle,Gomez Samantha,Celaya Elizabeth,Davis Ryan T.,Tankanzyan Ani,Nguyen Quy H.,Kessenbrock Kai,Zhao Da,Pervolarakis Nicholas.An In Vitro Vascularized Micro-Tumor Model of Human Colorectal Cancer Recapitulates In Vivo Drug Responses[EB/OL].(2025-03-28)[2025-08-29].https://www.biorxiv.org/content/10.1101/2020.03.03.973891.点此复制
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