Human Biopsies in Nanofibrillar Cellulose Hydrogel – A Novel Method for Long-term Tissue Culture
Human Biopsies in Nanofibrillar Cellulose Hydrogel – A Novel Method for Long-term Tissue Culture
Abstract Advanced 3D in vitro models are laborious to prepare and susceptible to unintentional design errors due to culture adaptations, cell immaturity, xenofactors or yet incomplete knowledge of the dynamics within tissues or materials. In order to acquire cost-efficient research material with intact in vivo composition, we developed novel tissue culture method with plant-derived scaffolding. Human skin-, foreskin- and glioblastoma multiforme biopsies were dissected mechanically and cultivated for 28 days in plant-derived nanofibrillar cellulose hydrogel. Comparative cultures were done using mouse sarcoma tumor –derived Matrigel?. Long-term preservation of cultivated tissues was evaluated against typical immunohistochemical biomarkers for each tissue type: skin tissues for cytokeratins 5/6, E-cadherin and vimentin for sustained tissue structures, and brain neoplasia for Olig2, S100, Nestin, NOTCH1, MAP2 and GFAP for preserved disease profile. Histological analysis from both culture conditions showed that until day 28, all cultivated biopsy types were able to sustain their characteristic protein expressions without signs of necrosis. We here conclude a novel tissue culture model in xeno-free 3D scaffolding, that can enable long-term sample storage in vitro, studies of human tumor tissues and their non-neoplastic microenvironment, and innovations in personalized medicine research.
Zalaudek Iris、Koivuniemi Raili、Paasonen Lauri、Singer Georg、von Campe Gord、Stallinger Alexander、Schrom Silke、Yliperttula Marjo、Haybaeck Johannes、Niklander Johanna、Rinner Beate、Kleinegger Florian、Liegl-Atzwanger Bernadette
Department of Dermatology and Venerology, University of TriesteDrug Research Program, Faculty of Pharmacy, University of HelsinkiUPM-Kymmene Corporation, UPM BiomedicalsDepartment of Pediatric and Adolescent Surgery, Medical University of GrazDepartment of Neurosurgery, Medical University of GrazDivision of Biomedical Research, Medical University of GrazDivision of Biomedical Research, Medical University of GrazDrug Research Program, Faculty of Pharmacy, University of HelsinkiDiagnostic and Research Center for Molecular Biomedicine, Institute of Pathology, Medical University of Graz||Institute of Pathology, Neuropathology and Molecular Pathology, Medical University of InnsbruckDrug Research Program, Faculty of Pharmacy, University of Helsinki||Division of Biomedical Research, Medical University of GrazDivision of Biomedical Research, Medical University of GrazDivision of Biomedical Research, Medical University of Graz||Diagnostic and Research Center for Molecular Biomedicine, Institute of Pathology, Medical University of GrazDiagnostic and Research Center for Molecular Biomedicine, Institute of Pathology, Medical University of Graz
医学研究方法基础医学细胞生物学
Zalaudek Iris,Koivuniemi Raili,Paasonen Lauri,Singer Georg,von Campe Gord,Stallinger Alexander,Schrom Silke,Yliperttula Marjo,Haybaeck Johannes,Niklander Johanna,Rinner Beate,Kleinegger Florian,Liegl-Atzwanger Bernadette.Human Biopsies in Nanofibrillar Cellulose Hydrogel – A Novel Method for Long-term Tissue Culture[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2021.11.22.466872.点此复制
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