Deep sequencing reveals Campylobacter in commercial meat chickens less than 8 days old
Deep sequencing reveals Campylobacter in commercial meat chickens less than 8 days old
Abstract Campylobacter from contaminated poultry meat is a major source of human gastroenteritis worldwide. To date, attempts to control this zoonotic infection with on-farm biosecurity measures have been inconsistent. A cornerstone of these efforts has been the detection of chicken infection with microbiological culture, which typically does not occur until birds are at least 21 days old. Using molecular methods for detecting Campylobacter presence, 16S microbiome analysis and deep sequencing of the Campylobacter porA gene, Campylobacter can be identified at very low levels in most or all flocks fewer than 8 days old. These young chicks exhibit a much greater diversity of porA types than older birds testing positive for Campylobacter by culture or qPCR. This suggests that, as the bacteria multiply sufficiently to be detected by culture methods, one or two strains, as indicated by porA type, dominate the infection. The findings that (i) most young chicks carry some Campylobacter and (ii) not all flocks become Campylobacter positive by culture, suggests that efforts to control infection should concentrate on how to maintain Campylobacter at low levels by the prevention of the overgrowth of single strains, which ultimately leads to the contamination of food.
Smith A.L.、Colles F.M.、Hedges S.J.、Dixon R.、Preston S.G.、Creach P.、Dawkins M.S.、Thornhill P.、Barfod K.K.、Maiden M.C.J.、Gebhardt-Henrich S.G.
The Peter Medawar Building for Pathogen Research, Department of Zoology, University of OxfordThe Peter Medawar Building for Pathogen Research, Department of Zoology, University of Oxford||NIHR Health Protection Research Unit in Gastrointestinal Infections, University of OxfordThe Peter Medawar Building for Pathogen Research, Department of Zoology, University of OxfordThe Peter Medawar Building for Pathogen Research, Department of Zoology, University of OxfordThe Peter Medawar Building for Pathogen Research, Department of Zoology, University of OxfordITAVIJohn Krebs Field Station, Department of Zoology, University of OxfordThe Peter Medawar Building for Pathogen Research, Department of Zoology, University of OxfordThe Peter Medawar Building for Pathogen Research, Department of Zoology, University of Oxford||Section for Experimental Animal Models, University of CopenhagenThe Peter Medawar Building for Pathogen Research, Department of Zoology, University of Oxford||NIHR Health Protection Research Unit in Gastrointestinal Infections, University of OxfordUniversity of Bern, Center for Proper Housing: Poultry and Rabbits, Division of Animal Welfare, University of Bern
微生物学医学研究方法预防医学
Smith A.L.,Colles F.M.,Hedges S.J.,Dixon R.,Preston S.G.,Creach P.,Dawkins M.S.,Thornhill P.,Barfod K.K.,Maiden M.C.J.,Gebhardt-Henrich S.G..Deep sequencing reveals Campylobacter in commercial meat chickens less than 8 days old[EB/OL].(2025-03-28)[2025-05-18].https://www.biorxiv.org/content/10.1101/2020.06.10.141978.点此复制
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