Bioengineering an in situ ovary (ISO) for fertility preservation
Bioengineering an in situ ovary (ISO) for fertility preservation
Female cancer patients who have undergone chemotherapy have an elevated risk of developing premature ovarian failure (POF) and infertility. Experimental approaches to treat iatrogenic infertility are evolving rapidly; however, there remain challenges and risks that have hindered clinical translation. To address these concerns, we developed an ovarian-specific extracellular matrix hydrogel to facilitate follicle delivery and establish an in situ ovary (ISO). We demonstrate sustainable engraftment, natural pregnancy and the birth of healthy pups after intraovarian microinjection of isolated exogenous follicles in a chemotherapy-induced POF mouse model. Our results suggest that the methods described could offer a minimally invasive alternative for the restoration of the ovarian reserve post-chemotherapy.
Orwig Kyle E.、Nolfi Alexis L.、Dadi Srujan、Case Zachary W.、D?ˉAmore Antonio、Iftikhar Aimon、Xian Ziyu、Steimer Sarah R.、Brown Bryan N.、Sukhwani Meena、Buckenmeyer Michael J.
Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of MedicineDepartment of Bioengineering, University of Pittsburgh||McGowan Institute for Regenerative Medicine, University of PittsburghDepartment of Bioengineering, University of PittsburghMcGowan Institute for Regenerative Medicine, University of PittsburghMcGowan Institute for Regenerative Medicine, University of Pittsburgh||Fondazione RiMEDDepartment of Bioengineering, University of Pittsburgh||McGowan Institute for Regenerative Medicine, University of PittsburghDepartment of Bioengineering, University of PittsburghDepartment of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of MedicineDepartment of Bioengineering, University of Pittsburgh||McGowan Institute for Regenerative Medicine, University of Pittsburgh||Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of MedicineDepartment of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of MedicineDepartment of Bioengineering, University of Pittsburgh||McGowan Institute for Regenerative Medicine, University of Pittsburgh
生物工程学妇产科学肿瘤学
Orwig Kyle E.,Nolfi Alexis L.,Dadi Srujan,Case Zachary W.,D?ˉAmore Antonio,Iftikhar Aimon,Xian Ziyu,Steimer Sarah R.,Brown Bryan N.,Sukhwani Meena,Buckenmeyer Michael J..Bioengineering an in situ ovary (ISO) for fertility preservation[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2020.01.03.893941.点此复制
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