Interrogation of RNA-protein interaction dynamics in bacterial growth
Interrogation of RNA-protein interaction dynamics in bacterial growth
Abstract Characterising RNA-protein interaction dynamics is fundamental to understand how bacteria respond to their environment. In this study, we have analysed the dynamics of 91% of the Escherichia coli expressed proteome and the RNA-interaction properties of 271 RNA-binding proteins (RBPs) at different growth phases. We find that 68% of RBPs differentially bind RNA across growth phases and characterise 17 previously unannotated proteins as bacterial RBPs including YfiF, a ncRNA-binding protein. While these new RBPs are mostly present in Proteobacteria, two of them have human orthologs in the form of mitochondrial proteins associated with rare metabolic disorders. Moreover, we reveal novel RBP functions for proteins such as the chaperone HtpG, a new stationary phase tRNA-binding protein. Altogether, we provide the first dynamic RBPome of a bacterium, showcasing how this approach can reveal the function of uncharacterised proteins, and identify critical RNA-protein interactions for cell growth which could inform new antimicrobial therapies.
Lilley Kathryn S.、Davey Karen、Ule Jernej、Thomas Gavin H.、Villanueva Eneko、Herman Reyme、Dawson Charlotte S.、Monti Mie、Willis Anne E.
Cambridge Centre for Proteomics, Department of Biochemistry, University of CambridgeThe Francis Crick Institute||UK Dementia Research Institute at King?ˉs College LondonThe Francis Crick Institute||UK Dementia Research Institute at King?ˉs College LondonDepartment of Biology, University of YorkCambridge Centre for Proteomics, Department of Biochemistry, University of CambridgeDepartment of Biology, University of YorkCambridge Centre for Proteomics, Department of Biochemistry, University of CambridgeMRC Toxicology Unit, University of Cambridge, University of CambridgeMRC Toxicology Unit, University of Cambridge, University of Cambridge
分子生物学微生物学生物科学研究方法、生物科学研究技术
Lilley Kathryn S.,Davey Karen,Ule Jernej,Thomas Gavin H.,Villanueva Eneko,Herman Reyme,Dawson Charlotte S.,Monti Mie,Willis Anne E..Interrogation of RNA-protein interaction dynamics in bacterial growth[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2023.07.03.547468.点此复制
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