Three dimensionally printed microstructured alginate scaffolds for neural tissue engineering
Three dimensionally printed microstructured alginate scaffolds for neural tissue engineering
Abstract The integration of scaffolds, signalling cues, and cellular components is essential in tissue engineering to create an in vivo equivalent environment that supports physiological function. Scaffolds provide mechanical reinforcement for cellular proliferation and differentiation while providing cues that instruct the development of cells during culture. Alginate (Alg) is a versatile biopolymer for scaffold engineering. However, due to a lack of intrinsic cell-binding sites, thus far, Alg must be functionalized for cellular adhesion. Here, we demonstrate proof-of-concept, bioactive additive-free, microstructured Alg (M-Alg) scaffolds for neuron culture. The M-Alg scaffold was formed by introducing tetrapod-shaped ZnO (t-ZnO) microparticles as structural templates in the Alg that were subsequently removed. These transparent, porous, additive-free Alg-based scaffolds with neuron affinity are promising for neuroregenerative and organoid- related research. HighlightsTetrapod-shaped ZnO (t-ZnO) microparticles are used as a template for the fabrication of open interconnected channels and textured surfaces in 3D printed microstructured alginate (M-Alg) scaffolds.Primary mouse cortical neurons seeded on the 3D printed M-Alg scaffolds show improved adhesion and maturation with extensive neural projections forming inside the scaffolds.
Reimers Armin、Groth Thomas、Cynis Holger、Adelung Rainer、Sch¨1tt Fabian、Poon Joyce K.S.、Brunk Michael G.K.、Sacher Wesley D.、Hietel Benjamin、Li Jianfeng、Willems Christian
Department of Materials Science, Kiel UniversityDepartment Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-WittenbergDepartment of Drug Design and Target Validation, Fraunhofer Institute for Cell Therapy and Immunology||Junior Research Group Faculty of Medicine, Martin Luther University Halle-WittenbergDepartment of Materials Science, Kiel UniversityDepartment of Materials Science, Kiel UniversityMax Planck Institute of Microstructure Physics||Max Planck-University of Toronto Centre for Neural Science and Technology||Department of Electrical and Computer Engineering, University of Toronto, 10 King?ˉs College RoadMax Planck Institute of Microstructure Physics||Max Planck-University of Toronto Centre for Neural Science and TechnologyMax Planck Institute of Microstructure Physics||Max Planck-University of Toronto Centre for Neural Science and TechnologyDepartment of Drug Design and Target Validation, Fraunhofer Institute for Cell Therapy and ImmunologyMax Planck Institute of Microstructure Physics||Max Planck-University of Toronto Centre for Neural Science and TechnologyDepartment Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg
生物工程学材料科学细胞生物学
Reimers Armin,Groth Thomas,Cynis Holger,Adelung Rainer,Sch¨1tt Fabian,Poon Joyce K.S.,Brunk Michael G.K.,Sacher Wesley D.,Hietel Benjamin,Li Jianfeng,Willems Christian.Three dimensionally printed microstructured alginate scaffolds for neural tissue engineering[EB/OL].(2025-03-28)[2025-09-01].https://www.biorxiv.org/content/10.1101/2024.04.23.590678.点此复制
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