The Rhodobacter sphaeroides methionine sulfoxide reductase MsrP can reduce R - and S -diastereomers of methionine sulfoxide from a broad-spectrum of protein substrates
The Rhodobacter sphaeroides methionine sulfoxide reductase MsrP can reduce R - and S -diastereomers of methionine sulfoxide from a broad-spectrum of protein substrates
Abstract Methionine (Met) is prone to oxidation and can be converted to Met sulfoxide (MetO), which exists as R- and S-diastereomers. MetO can be reduced back to Met by the ubiquitous methionine sulfoxide reductase (Msr) enzymes. Canonical MsrA and MsrB were shown as absolutely stereospecific for the reduction of S- and R-diastereomer, respectively. Recently, the molybdenum-containing protein MsrP from Escherichia coli was shown to be able to reduce MetO of periplasmic proteins without apparent stereospecificity. Here, we describe the substrate specificity of the Rhodobacter sphaeroides MsrP. Proteomics analysis coupled to enzymology approaches indicate that it reduces a broad spectrum of periplasmic oxidized proteins. Moreover, using model proteins, we demonstrated RsMsrP preferentially reduces unfolded oxidized proteins and we confirmed that this enzyme, like its E. coli homolog, can reduce both R- and S-diastereomers of MetO with similar efficiency.
Lemaire David、Arnoux Pascal、Pignol David、Grosse Sandrine、Tarrago Lionel、Alonso B¨|atrice、Armengaud Jean、Miotello Guylaine、Siponen Marina I.、Sabaty Monique
UMR 7265, Centre National de Recherche Scientifique||Aix Marseille Universit¨|, Marseille||Laboratoire des Interactions Prot¨|ine-M¨|tal, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), Commissariat ¨¤ l??Energie Atomique et aux Energies Alternatives (CEA)Laboratoire de Bio¨|nerg¨|tique Cellulaire, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), Commissariat ¨¤ l??Energie Atomique et aux Energies Alternatives (CEA)||UMR 7265, Centre National de Recherche Scientifique||Aix Marseille Universit¨|, MarseilleLaboratoire de Bio¨|nerg¨|tique Cellulaire, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), Commissariat ¨¤ l??Energie Atomique et aux Energies Alternatives (CEA)||UMR 7265, Centre National de Recherche Scientifique||Aix Marseille Universit¨|, MarseilleLaboratoire de Bio¨|nerg¨|tique Cellulaire, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), Commissariat ¨¤ l??Energie Atomique et aux Energies Alternatives (CEA)||UMR 7265, Centre National de Recherche Scientifique||Aix Marseille Universit¨|, MarseilleLaboratoire de Bio¨|nerg¨|tique Cellulaire, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), Commissariat ¨¤ l??Energie Atomique et aux Energies Alternatives (CEA)||UMR 7265, Centre National de Recherche Scientifique||Aix Marseille Universit¨|, MarseilleLaboratoire de Bio¨|nerg¨|tique Cellulaire, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), Commissariat ¨¤ l??Energie Atomique et aux Energies Alternatives (CEA)||UMR 7265, Centre National de Recherche Scientifique||Aix Marseille Universit¨|, MarseilleLaboratoire Innovations technologiques pour la D¨|tection et le Diagnostic (Li2D), Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRALaboratoire Innovations technologiques pour la D¨|tection et le Diagnostic (Li2D), Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRALaboratoire de Bio¨|nerg¨|tique Cellulaire, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), Commissariat ¨¤ l??Energie Atomique et aux Energies Alternatives (CEA)||UMR 7265, Centre National de Recherche Scientifique||Aix Marseille Universit¨|, MarseilleLaboratoire de Bio¨|nerg¨|tique Cellulaire, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), Commissariat ¨¤ l??Energie Atomique et aux Energies Alternatives (CEA)||UMR 7265, Centre National de Recherche Scientifique||Aix Marseille Universit¨|, Marseille
生物化学分子生物学微生物学
Lemaire David,Arnoux Pascal,Pignol David,Grosse Sandrine,Tarrago Lionel,Alonso B¨|atrice,Armengaud Jean,Miotello Guylaine,Siponen Marina I.,Sabaty Monique.The Rhodobacter sphaeroides methionine sulfoxide reductase MsrP can reduce R - and S -diastereomers of methionine sulfoxide from a broad-spectrum of protein substrates[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/243626.点此复制
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