Mutanofactin promotes bacterial adhesion and biofilm formation of cariogenic Streptococcus mutans
Mutanofactin promotes bacterial adhesion and biofilm formation of cariogenic Streptococcus mutans
Abstract Cariogenic Streptococcus mutans is known as a predominant etiological agent of dental caries due to its exceptional capacity in forming biofilms. From strains of S. mutans isolated from dental plaque, we here discover a polyketide/non-ribosomal peptide biosynthetic gene cluster, muf, which directly correlates with a strong biofilm-forming capability. We then identify the muf-associated bioactive product, mutanofactin-697 that contains a novel molecular scaffold, along with its biosynthetic logic. Further mode-of-action studies reveal mutanofactin-697 binds to S. mutans cells nonspecifically, increases bacterial hydrophobicity, and promotes bacterial adhesion and subsequent biofilm formation. Our findings provide the first example of a microbial secondary metabolite promoting biofilm formation via a physicochemical approach, highlighting the significance of secondary metabolism in mediating critical processes related to the development of dental caries.
Pan Aifei、Zeng Lin、Burne Robert A.、Li Zhong-Rui、Zhang Wenjun、Maboudian Roya、Sun Jin、Du Yongle、Qian Pei-Yuan
Department of Chemical and Biomolecular Engineering, University of California||State Key Laboratory for Manufacturing Systems Engineering, Xi?ˉan Jiaotong UniversityDepartment of Oral Biology, College of Dentistry, University of FloridaDepartment of Oral Biology, College of Dentistry, University of FloridaDepartment of Chemical and Biomolecular Engineering, University of CaliforniaDepartment of Chemical and Biomolecular Engineering, University of California||Chan Zuckerberg BiohubDepartment of Chemical and Biomolecular Engineering, University of CaliforniaDepartment of Ocean Science and Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guanzhou), The Hong Kong University of Science and TechnologyDepartment of Chemical and Biomolecular Engineering, University of CaliforniaDepartment of Ocean Science and Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guanzhou), The Hong Kong University of Science and Technology
微生物学分子生物学生物化学
Pan Aifei,Zeng Lin,Burne Robert A.,Li Zhong-Rui,Zhang Wenjun,Maboudian Roya,Sun Jin,Du Yongle,Qian Pei-Yuan.Mutanofactin promotes bacterial adhesion and biofilm formation of cariogenic Streptococcus mutans[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2020.08.22.262196.点此复制
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