Pyochelin biotransformation by Staphylococcus aureus shapes bacterial competition with Pseudomonas aeruginosa in polymicrobial infections
Pyochelin biotransformation by Staphylococcus aureus shapes bacterial competition with Pseudomonas aeruginosa in polymicrobial infections
Summary Pseudomonas aeruginosa and Staphylococcus aureus are among the most frequently isolated bacterial species from polymicrobial infections of cystic fibrosis patients and chronic wounds. We applied mass spectrometry guided interaction studies to determine how chemical interaction shapes the fitness and community structure during co-infection of these two pathogens. We demonstrate that S. aureus is equipped with an elegant mechanism to inactivate pyochelin via the yet uncharacterized methyltransferase Spm (staphylococcal pyochelin methyltransferase). Methylation of pyochelin abolishes the siderophore activity of pyochelin and significantly lowers pyochelin-mediated intracellular ROS production in S. aureus. In a murine wound co-infection model, a S. aureus mutant unable to methylate pyochelin shows significantly lower fitness as compared to its parental strain. Thus, Spm mediated pyochelin methylation is a novel mechanism to increase S. aureus survival during in vivo competition with P. aeruginosa.
Schilcher K.、Schurr M.、Zeiler M. J.、Jens J.、Melander C.、Keim K.、Horswill A.R.、Jenul C.、Phelan V.V.
Department of Immunology and Microbiology, School of Medicine, University of ColoradoDepartment of Immunology and Microbiology, School of Medicine, University of ColoradoDepartment of Chemistry and Biochemistry, University of Notre DameDepartment of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of ColoradoDepartment of Chemistry and Biochemistry, University of Notre DameDepartment of Immunology and Microbiology, School of Medicine, University of ColoradoDepartment of Immunology and Microbiology, School of Medicine, University of Colorado||Department of Veterans Affairs, Eastern Colorado Health Care SystemDepartment of Immunology and Microbiology, School of Medicine, University of ColoradoDepartment of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado
微生物学生物科学研究方法、生物科学研究技术基础医学
Schilcher K.,Schurr M.,Zeiler M. J.,Jens J.,Melander C.,Keim K.,Horswill A.R.,Jenul C.,Phelan V.V..Pyochelin biotransformation by Staphylococcus aureus shapes bacterial competition with Pseudomonas aeruginosa in polymicrobial infections[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2022.04.18.486787.点此复制
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