Synchronization between chloroplastic and cytosolic protein synthesis for photosynthesis complex assembly
Synchronization between chloroplastic and cytosolic protein synthesis for photosynthesis complex assembly
Summary Through symbiosis, subunits of chloroplastic complexes are encoded in distinct genomes in the nucleus and organelles. For plant cells to maintain the stoichiometry of subunits and respond to environmental cues, orchestration of the nuclear and organellar gene expression systems is an essential task. However, the mechanism maintaining chloroplastic complexes remains largely enigmatic. Here, we simultaneously assessed the translatomes of the chloroplast and the cytoplasm via ribosome profiling and revealed the differential mechanisms employed by these two systems to cope with acute light/dark transitions: in chloroplasts, translational regulation is employed, whereas in the cytoplasm, control of the mRNA abundance is employed. This strategy is widely conserved in land plants (Arabidopsis and the grass plant Brachypodium) and green algae (Chlamydomonas). The translational control in chloroplasts may be established on the basis of organelle symbiosis; the primitive chloroplast in Glaucophyta (Cyanophora) was found to have already acquired translational control, whereas cyanobacteria (Synechocystis) employ control of the mRNA abundance. Moreover, treatment with photosynthetic electron transport inhibitors indicated that reduced plastoquinones may trigger translational activation in chloroplasts. Our work reveals an early origin of coordination of chloroplast and nuclear/cytoplasmic gene expression upon light exposure.
Matsui Minami、Hashimoto Atsushi、Kawamoto Naohiro、Hirayama Takashi、Toh Hirotaka、Mochida Keiichi、Yoshida Minoru、Wakigawa Taisei、Fujita Tomoya、Kuriyama Tomoko、Iwasaki Shintaro、Matsuura-Suzuki Eriko、Kurihara Yukio、Hirose Yuu
Synthetic Genomics Research Group, RIKEN Center for Sustainable Resource ScienceChemical Genomics Research Group, RIKEN Center for Sustainable Resource ScienceRNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering ResearchGroup of Environmental Response System, Institute of Plant Science and Resources, Okayama UniversityRNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering ResearchBioproductivity Informatics Research Team, RIKEN Center for Sustainable Resource Science||Kihara Institute for Biological Research, Yokohama City University||Graduate School of Nanobioscience, Yokohama City University||School of Information and Data Sciences, Nagasaki University||Microalgae Resource Upcycling Research Laboratory, RIKEN Baton Zone Program, RIKEN Cluster for Science, Technology and Innovation HubChemical Genomics Research Group, RIKEN Center for Sustainable Resource Science||Office of University Professors, The University of Tokyo||Collaborative Research Institute for Innovative Microbiology, The University of TokyoRNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research||Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoRNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research||School of Life Science and Technology, Tokyo Institute of TechnologySynthetic Genomics Research Group, RIKEN Center for Sustainable Resource ScienceRNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research||School of Life Science and Technology, Tokyo Institute of TechnologyRNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering ResearchSynthetic Genomics Research Group, RIKEN Center for Sustainable Resource ScienceDepartment of Applied Chemistry and Life Science, Toyohashi University of Technology
分子生物学植物学生物化学
Matsui Minami,Hashimoto Atsushi,Kawamoto Naohiro,Hirayama Takashi,Toh Hirotaka,Mochida Keiichi,Yoshida Minoru,Wakigawa Taisei,Fujita Tomoya,Kuriyama Tomoko,Iwasaki Shintaro,Matsuura-Suzuki Eriko,Kurihara Yukio,Hirose Yuu.Synchronization between chloroplastic and cytosolic protein synthesis for photosynthesis complex assembly[EB/OL].(2025-03-28)[2025-04-24].https://www.biorxiv.org/content/10.1101/2024.05.03.592458.点此复制
评论