Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish
Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish
Abstract Macrophages and their precursor cells, monocytes, are the first line of defense of the body against foreign pathogens and tissue damage. Although the origins of macrophages are diverse, some common transcription factors (such as PU.1) are required to ensure proper development of monocytes/macrophages. Here we report that the deficiency of zbtb14, a transcription repressor gene belonging to ZBTB family, leads to an aberrant expansion of monocyte/macrophage population in zebrafish. Mechanistically, Zbtb14 functions as a negative regulator of pu.1, and SUMOylation on a conserved lysine is essential for the repression activity of Zbtb14. Moreover, a serine to phenylalanine mutation found in an acute myeloid leukemia (AML) patient could target ZBTB14 protein to autophagic degradation. Hence, ZBTB14 is a newly identified gene implicated in both normal and malignant myelopoiesis.
Deng Yun、Zhou Jun、Zhu Jun、de Th¨| Hugues、Liu Xiaohui、Yuan Hao、Wang Haihong、Xu Jin
Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine||CNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences and Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineShanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine||CNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences and Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineCNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences and Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine||Universit ¨| de Paris 7/INSERM/CNRS UMR 944/7212, Equipe Labellis ¨| e Ligue Nationale Contre le Cancer, H?pital St. LouisCNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences and Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine||Universit ¨| de Paris 7/INSERM/CNRS UMR 944/7212, Equipe Labellis ¨| e Ligue Nationale Contre le Cancer, H?pital St. LouisShanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine||CNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences and Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineShanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine||CNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences and Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineShanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine||CNRS-LIA Hematology and Cancer, Sino-French Research Center for Life Sciences and Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineLaboratory of Immunology and Regeneration, School of Medicine, South China University of Technology
基础医学分子生物学生物科学研究方法、生物科学研究技术
Deng Yun,Zhou Jun,Zhu Jun,de Th¨| Hugues,Liu Xiaohui,Yuan Hao,Wang Haihong,Xu Jin.Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish[EB/OL].(2025-03-28)[2025-06-03].https://www.biorxiv.org/content/10.1101/2022.07.03.498625.点此复制
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