Spindle scaling is governed by cell boundary regulation of microtubule nucleation
Spindle scaling is governed by cell boundary regulation of microtubule nucleation
Abstract Cellular organelles such as the mitotic spindle adjust their size to the dimensions of the cell. It is widely understood that spindle scaling is governed by regulation of microtubule polymerization. Here we use quantitative microscopy in living zebrafish embryos and Xenopus egg extracts in combination with theory to show that microtubule polymerization dynamics are insufficient to scale spindles and only contribute below a critical cell size. In contrast, microtubule nucleation governs spindle scaling for all cell sizes. We show that this hierarchical regulation arises from the partitioning of a nucleation inhibitor to the cell membrane. Our results reveal that cells differentially regulate microtubule number and length using distinct geometric cues to maintain a functional spindle architecture over a large range of cell sizes.
Golfier Stefan、Decker Franziska、Ishihara Keisuke、Rieckhoff Elisa Maria、Brugu¨|s Jan、Elsner Maria、Berndt Frederic
Max Planck Institute of Molecular Cell Biology and Genetics||Max Planck Institute for the Physics of Complex Systems||Centre for Systems Biology Dresden||Cluster of Excellence Physics of LifeMax Planck Institute of Molecular Cell Biology and Genetics||Max Planck Institute for the Physics of Complex Systems||Centre for Systems Biology Dresden||Cluster of Excellence Physics of LifeMax Planck Institute of Molecular Cell Biology and Genetics||Max Planck Institute for the Physics of Complex Systems||Centre for Systems Biology Dresden||Cluster of Excellence Physics of LifeMax Planck Institute of Molecular Cell Biology and Genetics||Max Planck Institute for the Physics of Complex Systems||Centre for Systems Biology Dresden||Cluster of Excellence Physics of LifeMax Planck Institute of Molecular Cell Biology and Genetics||Max Planck Institute for the Physics of Complex Systems||Centre for Systems Biology Dresden||Cluster of Excellence Physics of LifeMax Planck Institute of Molecular Cell Biology and Genetics||Max Planck Institute for the Physics of Complex Systems||Centre for Systems Biology Dresden||Cluster of Excellence Physics of LifeMax Planck Institute of Molecular Cell Biology and Genetics||Max Planck Institute for the Physics of Complex Systems||Centre for Systems Biology Dresden||Cluster of Excellence Physics of Life
细胞生物学生物科学现状、生物科学发展生物科学研究方法、生物科学研究技术
Golfier Stefan,Decker Franziska,Ishihara Keisuke,Rieckhoff Elisa Maria,Brugu¨|s Jan,Elsner Maria,Berndt Frederic.Spindle scaling is governed by cell boundary regulation of microtubule nucleation[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2020.06.15.136937.点此复制
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