Persistence and detection of anti-SARS-CoV-2 antibodies: immunoassay heterogeneity and implications for serosurveillance
Persistence and detection of anti-SARS-CoV-2 antibodies: immunoassay heterogeneity and implications for serosurveillance
Abstract Serologic studies have been critical in tracking the evolution of the COVID-19 pandemic. The reliability of serologic studies for quantifying the proportion of the population that have been infected depends on the extent of antibody decay as well as on assay performance in detecting both recent and older infections. Data on anti-SARS-CoV-2 antibodies persistence remain sparse, especially from infected individuals with few to no symptoms. In a cohort of mostly mild/asymptomatic SARS-CoV-2-infected individuals tested with three widely-used immunoassays, antibodies persisted for at least 8 months after infection, although detection depended on immunoassay choice, with one of them missing up to 40% of past infections. Simulations reveal that without appropriate adjustment for time-varying assay sensitivity, seroprevalence surveys may underestimate infection rates. As the immune landscape becomes more complex with naturally-infected and vaccinated individuals, assay choice and appropriate assay-performance-adjustment will become even more important for the interpretation of serologic studies.
Yerly Sabine、Meyer Benjamin、Trono Didier、Stringhini Silvia、Eckerle Isabella、Azman Andrew S、Vuilleumier Nicolas、Guessous Idris、Kaiser Laurent、Zaballa Mar¨aa-Eugenia、for the Specchio-COVID19 Study Group、Pittet Didier、Andrey Diego O.、Balavoine Jean-Fran?ois、Perez-Saez Javier、Chappuis Fran?ois、Kherad Omar
Laboratory of Virology, Department of Diagnostics, Geneva University Hospitals and Faculty of MedicineCentre for Vaccinology, Department of Pathology and Immunology, University of GenevaSchool of Life Sciences, Ecole Polytechnique F¨|d¨|rale de Lausanne (EPFL)Unit of Population Epidemiology, Division of Primary Care Medicine, Geneva University Hospitals||Department of Health and Community Medicine, Faculty of Medicine, University of Geneva||University Center for General Medicine and Public Health, University of LausanneLaboratory of Virology, Department of Diagnostics, Geneva University Hospitals and Faculty of Medicine||Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine||Geneva Centre for Emerging Viral Diseases, Geneva University Hospitals and Faculty of MedicineDepartment of Epidemiology, Johns Hopkins Bloomberg School of Public Health||Unit of Population Epidemiology, Division of Primary Care Medicine, Geneva University Hospitals||Institute of Global Health, Faculty of Medicine, University of GenevaDivision of Laboratory Medicine, Department of Diagnostics, Geneva University Hospitals and Faculty of Medicine||Department of Medicine, Faculty of Medicine, University of GenevaDivision and Department of Primary Care Medicine, Geneva University Hospitals||Department of Health and Community Medicine, Faculty of Medicine, University of GenevaLaboratory of Virology, Department of Diagnostics, Geneva University Hospitals and Faculty of Medicine||Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of Medicine||Geneva Centre for Emerging Viral Diseases, Geneva University Hospitals and Faculty of MedicineUnit of Population Epidemiology, Division of Primary Care Medicine, Geneva University HospitalsInfection Control Program and World Health Organization Collaborating Center on Patient Safety, Geneva University Hospitals and Faculty of MedicineDivision of Laboratory Medicine, Department of Diagnostics, Geneva University Hospitals and Faculty of Medicine||Division of Infectious Diseases, Department of Medicine, Geneva University Hospitals and Faculty of MedicineDepartment of Medicine, Faculty of Medicine, University of GenevaDepartment of Epidemiology, Johns Hopkins Bloomberg School of Public Health||Institute of Global Health, Faculty of Medicine, University of GenevaDivision and Department of Primary Care Medicine, Geneva University HospitalsDivision of Internal Medicine, H?pital de la Tour and Faculty of Medicine
医学研究方法预防医学基础医学
Yerly Sabine,Meyer Benjamin,Trono Didier,Stringhini Silvia,Eckerle Isabella,Azman Andrew S,Vuilleumier Nicolas,Guessous Idris,Kaiser Laurent,Zaballa Mar¨aa-Eugenia,for the Specchio-COVID19 Study Group,Pittet Didier,Andrey Diego O.,Balavoine Jean-Fran?ois,Perez-Saez Javier,Chappuis Fran?ois,Kherad Omar.Persistence and detection of anti-SARS-CoV-2 antibodies: immunoassay heterogeneity and implications for serosurveillance[EB/OL].(2025-03-28)[2025-05-11].https://www.medrxiv.org/content/10.1101/2021.03.16.21253710.点此复制
评论