A molecular pore spans the double membrane of the coronavirus replication organelle
A molecular pore spans the double membrane of the coronavirus replication organelle
Coronavirus genome replication is associated with virus-induced cytosolic double-membrane vesicles, which may provide a tailored micro-environment for viral RNA synthesis in the infected cell. However, it is unclear how newly synthesized genomes and mRNAs can travel from these sealed replication compartments to the cytosol to ensure their translation and the assembly of progeny virions. Here, using cellular electron cryo-microscopy, we unveiled a molecular pore complex that spans both membranes of the double-membrane vesicle and would allow export of RNA to the cytosol. A hexameric assembly of a large viral transmembrane protein was found to form the core of the crown-shaped complex. This coronavirus-specific structure likely plays a critical role in coronavirus replication and thus constitutes a novel drug target
Wolff Georg、Zevenhoven-Dobbe Jessika C.、Laugks Ulrike、de Jong Anja W. M.、Agard David A.、Snijder Eric J.、Koning Roman I.、Gr¨1newald Kay、Koster Abraham J.、Limpens Ronald W.A.L.、B¨￠rcena Montserrat、Zheng Shawn
Department of Cell and Chemical Biology, Section Electron Microscopy, Leiden University Medical CenterDepartment of Medical Microbiology, Molecular Virology Laboratory, Leiden University Medical CenterDepartment of Structural Cell Biology of Viruses, Centre for Structural Systems Biology, Heinrich Pette Institute, Leibnitz Institute of Experimental Virology, University of HamburgDepartment of Cell and Chemical Biology, Section Electron Microscopy, Leiden University Medical CenterDepartment of Biochemistry and Biophysics, University of California San FranciscoDepartment of Medical Microbiology, Molecular Virology Laboratory, Leiden University Medical CenterDepartment of Cell and Chemical Biology, Section Electron Microscopy, Leiden University Medical CenterDepartment of Structural Cell Biology of Viruses, Centre for Structural Systems Biology, Heinrich Pette Institute, Leibnitz Institute of Experimental Virology, University of Hamburg||Department of Chemistry, MIN Faculty, Universit?t HamburgDepartment of Cell and Chemical Biology, Section Electron Microscopy, Leiden University Medical CenterDepartment of Cell and Chemical Biology, Section Electron Microscopy, Leiden University Medical CenterDepartment of Cell and Chemical Biology, Section Electron Microscopy, Leiden University Medical CenterDepartment of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California San Francisco
分子生物学细胞生物学
Wolff Georg,Zevenhoven-Dobbe Jessika C.,Laugks Ulrike,de Jong Anja W. M.,Agard David A.,Snijder Eric J.,Koning Roman I.,Gr¨1newald Kay,Koster Abraham J.,Limpens Ronald W.A.L.,B¨￠rcena Montserrat,Zheng Shawn.A molecular pore spans the double membrane of the coronavirus replication organelle[EB/OL].(2025-03-28)[2025-05-01].https://www.biorxiv.org/content/10.1101/2020.06.25.171686.点此复制
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