A small interfering RNA (siRNA) database for SARS-CoV-2
A small interfering RNA (siRNA) database for SARS-CoV-2
ABSTRACT Coronavirus disease 2019 (COVID-19) rapidly transformed into a global pandemic, for which a demand for developing antivirals capable of targeting the SARS-CoV-2 RNA genome and blocking the activity of its genes has emerged. In this work, we propose a database of SARS-CoV-2 targets for siRNA approaches, aiming to speed the design process by providing a broad set of possible targets and siRNA sequences. Beyond target sequences, it also displays more than 170 features, including thermodynamic information, base context, target genes and alignment information of sequences against the human genome, and diverse SARS-CoV-2 strains, to assess whether siRNAs targets bind or not off-target sequences. This dataset is available as a set of four tables in a single spreadsheet file, each table corresponding to sequences of 18, 19, 20, and 21 nucleotides length, respectively, aiming to meet the diversity of technology and expertise among labs around the world concerning siRNAs design of varied sizes, more specifically between 18 and 21nt length. We hope that this database helps to speed the development of new target antivirals for SARS-CoV-2, contributing to more rapid and effective responses to the COVID-19 pandemic.
dos Santos Sidney Emanuel Batista、Stransky Beatriz、Khayat Andr¨| Salim、de Assump??o Paulo Pimentel、de Souza Jorge Estefano Santana、Medeiros In¨￠cio Gomes
Instituto de Ci¨oncias Biol¨?gicas, Universidade Federal do Par¨￠||N¨2cleo de Pesquisas em Oncologia, Universidade Federal do Par¨￠Bioinformatics Multidisciplinary Environment (BioME), Metr¨?pole Digital Institute, Federal University of Rio Grande do Norte||Biomedical Engineering Department, Center of Technology, Federal University of Rio Grande do NorteInstituto de Ci¨oncias Biol¨?gicas, Universidade Federal do Par¨￠N¨2cleo de Pesquisas em Oncologia, Universidade Federal do Par¨￠Bioinformatics Graduate Program, Metr¨?pole Digital Institute, Federal University of Rio Grande do Norte||Bioinformatics Multidisciplinary Environment (BioME), Metr¨?pole Digital Institute, Federal University of Rio Grande do NorteBioinformatics Graduate Program, Metr¨?pole Digital Institute, Federal University of Rio Grande do Norte||Instituto do C¨|rebro, Federal University of Rio Grande do Norte
生物科学研究方法、生物科学研究技术基础医学分子生物学
dos Santos Sidney Emanuel Batista,Stransky Beatriz,Khayat Andr¨| Salim,de Assump??o Paulo Pimentel,de Souza Jorge Estefano Santana,Medeiros In¨￠cio Gomes.A small interfering RNA (siRNA) database for SARS-CoV-2[EB/OL].(2025-03-28)[2025-05-07].https://www.biorxiv.org/content/10.1101/2020.09.30.321596.点此复制
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