The TPLATE subunit is essential for structural assembly of the endocytic TSET complex
The TPLATE subunit is essential for structural assembly of the endocytic TSET complex
Summary All eukaryotic cells rely on endocytosis to regulate the plasma membrane proteome and lipidome. Most eukaryotic groups, with the exception of fungi and animals, have retained the evolutionary ancient TSET complex as a regulator of endocytosis. Despite the presence of similar building blocks in TSET, compared to other coatomer complexes, structural insight into this adaptor complex is lacking. Here, we elucidate the molecular architecture of the octameric plant TSET complex (TPLATE complex/TPC) using an integrative structural approach. This allowed us to describe a plant-specific connection between the TML subunit and the AtEH/Pan1 proteins and show a direct interaction between the complex and the plasma membrane without the need for any additional protein factors. Furthermore, we identify the appendage of TPLATE as crucial for complex assembly. Structural elucidation of this ancient adaptor complex vastly advances our functional as well as evolutionary insight into the process of endocytosis. Graphical abstractbiorxiv;2020.08.13.249276v3/UFIG1F1ufig1
Mylle Evelien、Pleskot Roman、Yperman Klaas、Merceron Romain、Nolf Jonah、Savvides Savvas N.、Wang Jie、Grones Peter、Potocky Martin、Eeckhout Dominique、De Jaeger Geert、Vu Lam Dai、De Rybel Bert、De Bruyn Pieter、Mor Eliana、Vandorpe Michael、Kraus Michael、Van Damme Daniel、Loris Remy、Winkler Joanna
Ghent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyDepartment of Biochemistry and Microbiology, Ghent University||VIB Center for Inflammation ResearchGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyDepartment of Biochemistry and Microbiology, Ghent University||VIB Center for Inflammation ResearchGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyInstitute of Experimental Botany, Academy of Sciences of the Czech RepublicGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyVrije Universiteit Brussel, Structural Biology Brussels, Department of Biotechnology||VIB-VUB Center for Structural Biology, Structural Biology Research Center, Molecular Recognition UnitGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyVrije Universiteit Brussel, Structural Biology Brussels, Department of Biotechnology||VIB-VUB Center for Structural Biology, Structural Biology Research Center, Molecular Recognition UnitGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems Biology
细胞生物学分子生物学植物学
Mylle Evelien,Pleskot Roman,Yperman Klaas,Merceron Romain,Nolf Jonah,Savvides Savvas N.,Wang Jie,Grones Peter,Potocky Martin,Eeckhout Dominique,De Jaeger Geert,Vu Lam Dai,De Rybel Bert,De Bruyn Pieter,Mor Eliana,Vandorpe Michael,Kraus Michael,Van Damme Daniel,Loris Remy,Winkler Joanna.The TPLATE subunit is essential for structural assembly of the endocytic TSET complex[EB/OL].(2025-03-28)[2025-05-09].https://www.biorxiv.org/content/10.1101/2020.08.13.249276.点此复制
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