WHotLAMP: A simple, inexpensive, and sensitive molecular test for the detection of SARS-CoV-2 in saliva
WHotLAMP: A simple, inexpensive, and sensitive molecular test for the detection of SARS-CoV-2 in saliva
Abstract Despite the development of effective vaccines against SARS-CoV-2, epidemiological control of the virus is still challenging due to slow vaccine rollouts, incomplete vaccine protection to current and emerging variants, and unwillingness to get vaccinated. Therefore, frequent testing of individuals to identify early SARS-CoV-2 infections, contact-tracing and isolation strategies remain crucial to mitigate viral spread. Here, we describe WHotLAMP, a rapid molecular test to detect SARS-CoV-2 in saliva. WHotLAMP is simple to use, highly sensitive (3.6 viral RNA copies per microliter of saliva) and specific, as well as inexpensive, making it ideal for frequent screening. Moreover, WHotLAMP does not require toxic chemicals or specialized equipment and thus can be performed in point-of-care settings, and may also be adapted for resource-limited environments or home use. While applied here to SARS-CoV-2, WHotLAMP can be modified to detect other pathogens, making it adaptable for other diagnostic assays, including for use in future outbreaks.
Fiederling Felix、Andolfatto Peter、Tabachnik Tanya、D?ˉArmiento Jeanine、Pinharanda Ana、Litwin-Kumar Ashok、Thopte Urvashi、Kosmidis Stylianos、Noro Barbara、Rodrigues-Vaz Ines、Shayya Hani、Stearns Kyle、Enikolopov Armen、Ng David、Goldklang Monica、Cannavo Enrico、Bendesky Andres、Peterka Darcy S.、Vogt Merly C.
Zuckerman Mind Brain Behavior Institute, Columbia UniversityDepartment of Biological Sciences, Columbia UniversityZuckerman Mind Brain Behavior Institute, Columbia UniversityDepartment of Anesthesiology, Columbia University Irving Medical Center||Center for LAM and Rare Lung Diseases, Columbia University Irving Medical CenterDepartment of Biological Sciences, Columbia UniversityZuckerman Mind Brain Behavior Institute, Columbia UniversityZuckerman Mind Brain Behavior Institute, Columbia UniversityZuckerman Mind Brain Behavior Institute, Columbia UniversityZuckerman Mind Brain Behavior Institute, Columbia UniversityZuckerman Mind Brain Behavior Institute, Columbia UniversityZuckerman Mind Brain Behavior Institute, Columbia UniversityDepartment of Anesthesiology, Columbia University Irving Medical CenterZuckerman Mind Brain Behavior Institute, Columbia UniversityDepartment of Anesthesiology, Columbia University Irving Medical Center||Center for LAM and Rare Lung Diseases, Columbia University Irving Medical CenterZuckerman Mind Brain Behavior Institute, Columbia UniversityZuckerman Mind Brain Behavior Institute, Columbia University||Department of Ecology, Evolution and Environmental Biology, Columbia UniversityZuckerman Mind Brain Behavior Institute, Columbia UniversityDepartment of Biological Sciences, Columbia University||Howard Hughes Medical Institute, Columbia University
医学研究方法预防医学分子生物学
Fiederling Felix,Andolfatto Peter,Tabachnik Tanya,D?ˉArmiento Jeanine,Pinharanda Ana,Litwin-Kumar Ashok,Thopte Urvashi,Kosmidis Stylianos,Noro Barbara,Rodrigues-Vaz Ines,Shayya Hani,Stearns Kyle,Enikolopov Armen,Ng David,Goldklang Monica,Cannavo Enrico,Bendesky Andres,Peterka Darcy S.,Vogt Merly C..WHotLAMP: A simple, inexpensive, and sensitive molecular test for the detection of SARS-CoV-2 in saliva[EB/OL].(2025-03-28)[2025-08-02].https://www.medrxiv.org/content/10.1101/2021.06.17.21259050.点此复制
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