The crystal structure of nsp10-nsp16 heterodimer from SARS-CoV-2 in complex with S-adenosylmethionine
The crystal structure of nsp10-nsp16 heterodimer from SARS-CoV-2 in complex with S-adenosylmethionine
Abstract SARS-CoV-2 is a member of the coronaviridae family and is the etiological agent of the respiratory Coronavirus Disease 2019. The virus has spread rapidly around the world resulting in over two million cases and nearly 150,000 deaths as of April 17, 2020. Since no treatments or vaccines are available to treat COVID-19 and SARS-CoV-2, respiratory complications derived from the infections have overwhelmed healthcare systems around the world. This virus is related to SARS-CoV-1, the virus that caused the 2002-2004 outbreak of Severe Acute Respiratory Syndrome. In January 2020, the Center for Structural Genomics of Infectious Diseases implemented a structural genomics pipeline to solve the structures of proteins essential for coronavirus replication-transcription. Here we show the first structure of the SARS-CoV-2 nsp10-nsp16 2’-O-methyltransferase complex with S-adenosylmethionine at a resolution of 1.80 ?. This heterodimer complex is essential for capping viral mRNA transcripts for efficient translation and to evade immune surveillance.
Kim Youngchang、Rosas-Lemus Monica、Inniss Nicole L.、Jedrzejczak Robert、Endres Michael、Godzik Adam、Maltseva Natalia I.、Shuvalova Ludmilla、Satchell Karla J. F.、Minasov George、Joachimiak Andrzej、Jaroszewski Lukasz、Kiryukhina Olga、Wiersum Grant
Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine||Consortium for Advanced Science and Engineering, University of Chicago||Structural Biology Center, X-ray Science Division, Argonne National LaboratoryDepartment of Microbiology-Immunology, Northwestern University, Feinberg School of Medicine||Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of MedicineDepartment of Microbiology-Immunology, Northwestern University, Feinberg School of Medicine||Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of MedicineCenter for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine||Consortium for Advanced Science and Engineering, University of Chicago||Structural Biology Center, X-ray Science Division, Argonne National LaboratoryCenter for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine||Consortium for Advanced Science and Engineering, University of ChicagoCenter for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine||Department of Biomedical Sciences, University of California, Riverside School of MedicineCenter for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine||Consortium for Advanced Science and Engineering, University of ChicagoDepartment of Microbiology-Immunology, Northwestern University, Feinberg School of Medicine||Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of MedicineDepartment of Microbiology-Immunology, Northwestern University, Feinberg School of Medicine||Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of MedicineDepartment of Microbiology-Immunology, Northwestern University, Feinberg School of Medicine||Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of MedicineCenter for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine||Consortium for Advanced Science and Engineering, University of Chicago||Structural Biology Center, X-ray Science Division, Argonne National Laboratory||Department of Biochemistry and Molecular Biology, University of ChicagoCenter for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine||Department of Biomedical Sciences, University of California, Riverside School of MedicineDepartment of Microbiology-Immunology, Northwestern University, Feinberg School of Medicine||Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of MedicineDepartment of Microbiology-Immunology, Northwestern University, Feinberg School of Medicine||Center for Structural Genomics of Infectious Diseases, Northwestern University, Feinberg School of Medicine
基础医学分子生物学微生物学
Kim Youngchang,Rosas-Lemus Monica,Inniss Nicole L.,Jedrzejczak Robert,Endres Michael,Godzik Adam,Maltseva Natalia I.,Shuvalova Ludmilla,Satchell Karla J. F.,Minasov George,Joachimiak Andrzej,Jaroszewski Lukasz,Kiryukhina Olga,Wiersum Grant.The crystal structure of nsp10-nsp16 heterodimer from SARS-CoV-2 in complex with S-adenosylmethionine[EB/OL].(2025-03-28)[2025-05-13].https://www.biorxiv.org/content/10.1101/2020.04.17.047498.点此复制
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