Modeling small RNA competition in C. elegans

Website SummarySmall RNAs are important regulators of gene expression; however, the relationship between small RNAs is poorly understood. Studying the crosstalk between small RNA pathways can help understand how gene expression is regulated. One hypothesis suggests that small RNA competition arises from limited enzymatic resources. Therefore, a model was created in order to gain insights into this competition. AbstractSmall RNAs have been determined to have an essential role in gene regulation. However, competition between small RNAs is a poorly understood aspect of small RNA dynamics. Recent evidence has suggested that competition between small RNA pathways arises from a scarcity of common resources essential for small RNA activity. In order to understand how competition affects small RNAs in C. elegans, a system of differential equations was used. The model recreates normal behavior of small RNAs and uses random sampling in order to determine the coefficients of competition for each small RNA class. The model includes endogenous small-interfering RNAs (endo-siRNA), exogenous small-interfering RNAs (exo-siRNA), and microRNAs (miRNA). The model predicts that exo-siRNAs is dominated by competition between endo-siRNAs and miRNAs. Furthermore, the model predicts that competition is required for normal levels of endogenous small RNAs to be maintained. Although the model makes several assumptions about cell dynamics, the model is still useful in order to understand competition between small RNA pathways.