Regulatory elements can be essential for maintaining broad chromatin organization and cell viability
Regulatory elements can be essential for maintaining broad chromatin organization and cell viability
ABSTRACT Increasing evidence shows that promoters and enhancers could be related to 3D chromatin structure, thus affecting cellular functions. Except for functioning through the canonical chromatin loops formed by promoters and enhancers, their roles in maintaining broad chromatin organization have not been well studied. Here, we focused on the active promoters/enhancers (referred to as hotspots) predicted to form many 3D contacts with other active promoters/enhancers, and identified dozens of loci critical for cell survival. While the essentiality of hotspots is not resulted from their association with essential genes, deletion of an essential hotspot could lead to change of broad chromatin organization and expressions of distal genes. We demonstrated that multiple affected genes that are individually non-essential could have synergistic effects to cause cell death.
Xu Ping、Zhao Ying、Wang Wei、Guo Yu、Ding Bo、Wu Peiyao、Liu Ying、Pan Qian、Chen Zhao、Liu Zhiheng、Zheng Lina、Wei Wensheng
Biomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, Peking University Genome Editing Research Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking UniversityDepartment of Chemistry and Biochemistry, University of CaliforniaDepartment of Chemistry and Biochemistry, University of California||Bioinformatics and Systems Biology program, University of California||Department of Cellular and Molecular Medicine, University of CaliforniaBiomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, Peking University Genome Editing Research Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking UniversityDepartment of Chemistry and Biochemistry, University of CaliforniaDepartment of Chemistry and Biochemistry, University of CaliforniaBiomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, Peking University Genome Editing Research Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking UniversityBiomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, Peking University Genome Editing Research Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking UniversityDepartment of Chemistry and Biochemistry, University of CaliforniaBiomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, Peking University Genome Editing Research Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University||Academy for Advanced Interdisciplinary Studies, Peking UniversityBioinformatics and Systems Biology program, University of CaliforniaBiomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, Peking University Genome Editing Research Center, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University
分子生物学细胞生物学遗传学
Xu Ping,Zhao Ying,Wang Wei,Guo Yu,Ding Bo,Wu Peiyao,Liu Ying,Pan Qian,Chen Zhao,Liu Zhiheng,Zheng Lina,Wei Wensheng.Regulatory elements can be essential for maintaining broad chromatin organization and cell viability[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2020.12.13.422554.点此复制
评论