Ratiometric control of two microbial populations via a dual chamber bioreactor
Ratiometric control of two microbial populations via a dual chamber bioreactor
Abstract Maintaining stable coexistence in microbial consortia, particularly when one species grows faster than another (i.e. the species are non-complementary), poses significant challenges. We introduce a novel control architecture that employs two bioreactors. In this system, the slower-growing species is cultivated separately before being introduced into the main mixing chamber. We analyze the open-loop dynamics of this setup and propose a switching feedback mechanism that controls the dilution rates to ensure robust regulation of population density and composition within the microbial consortium. Validated in silico using parameters from real experiments, our approach demonstrates effective and robust maintenance of microbial balance across various strains without requiring genetic modifications.
Russo Giovanni、Salzano Davide、Fiore Davide、Brancato Sara Maria、di Bernardo Mario
Department of Information Engineering, Electrical Engineering and Applied Mathematics, University of SalernoScuola Superiore MeridionaleDepartment of Mathematics and Applications ?°R. Caccioppoli?±, University of Naples Federico IIDepartment of Electrical Engineering and Information Technology, University of Naples Federico IIDepartment of Electrical Engineering and Information Technology, University of Naples Federico II||Scuola Superiore Meridionale
生物工程学自动化技术、自动化技术设备
Russo Giovanni,Salzano Davide,Fiore Davide,Brancato Sara Maria,di Bernardo Mario.Ratiometric control of two microbial populations via a dual chamber bioreactor[EB/OL].(2025-03-28)[2025-05-09].https://www.biorxiv.org/content/10.1101/2024.03.08.584056.点此复制
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