Held out wings RNA binding activity in the cytoplasm during early spermatogenesis
Held out wings RNA binding activity in the cytoplasm during early spermatogenesis
Abstract Held out wings (HOW) is an RNA-binding protein essential for spermatogenesis in Drosophila melanogaster. HOW is a signal transduction and activation of RNA (STAR) protein, regulating post-transcriptional gene expression. The characteristics of RNA-binding by the conserved short cytoplasmic isoform, HOW(S), are unknown. In vivo RIP-seq identified 121 novel transcripts bound by HOW(S) in germ stem cells and spermatogonia, many with signal transduction functions. (A/G/U)CUAAC motifs were enriched in 3’-UTRs and GCG(A/U)G in 5’-UTRs. HOW binds with high-affinity to sites containing CUAAC motifs from lola and hipk mRNAs. This study provides new insight into STAR protein-RNA interactions and functions in spermatogenesis.
Agapiou Michaela、Edwards Thomas A.、Aspden Julie L.、Bretman Amanda、Hopes Tayah、Hobor Fruzsina
School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds||LeedsOmics, University of LeedsSchool of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds||Astbury Centre for Structural Molecular Biology, University of LeedsSchool of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds||LeedsOmics, University of LeedsSchool of Biology, Faculty of Biological Sciences, University of LeedsSchool of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds||LeedsOmics, University of LeedsSchool of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds||Astbury Centre for Structural Molecular Biology, University of Leeds
分子生物学遗传学细胞生物学
Agapiou Michaela,Edwards Thomas A.,Aspden Julie L.,Bretman Amanda,Hopes Tayah,Hobor Fruzsina.Held out wings RNA binding activity in the cytoplasm during early spermatogenesis[EB/OL].(2025-03-28)[2025-06-05].https://www.biorxiv.org/content/10.1101/2022.05.19.492257.点此复制
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