Miro1-dependent Mitochondrial Dynamics in Parvalbumin Interneurons
Miro1-dependent Mitochondrial Dynamics in Parvalbumin Interneurons
SUMMARY The spatiotemporal distribution of mitochondria is crucial for precise ATP provision and calcium buffering required to support neuronal signaling. Fast-spiking GABAergic interneurons expressing parvalbumin (PV) have a high mitochondrial content reflecting their large energy utilization. The importance for correct trafficking and precise mitochondrial positioning remains poorly elucidated in inhibitory neurons. Miro1 is a Ca2+-sensing adaptor protein that links mitochondria to the trafficking apparatus, for their microtubule-dependent transport along axons and dendrites, in order to meet the metabolic and Ca2+-buffering requirements of the cell. Here, we explore the role of Miro1 in parvalbumin interneurons and how changes in mitochondrial trafficking could alter brain network activity. By employing live and fixed imaging, we found that the impairments in Miro1-directed trafficking in PV+ interneurons altered their mitochondrial distribution and axonal arborization. These changes were accompanied by an increase in the ex vivo hippocampal γ-oscillation (30 – 80 Hz) frequency and promoted anxiolysis. Our findings show that precise regulation of mitochondrial dynamics in PV+ interneurons is crucial for proper neuronal signaling and network synchronization.
Mann Edward O.、Arancibia-Carcamo I. Lorena、Lopez-Domenech Guillermo、Salinas Patricia C.、Kittler Josef T.、Kontou Georgina、Higgs Nathalie F.、Podpolny Marina、Szulc Blanka R.、Antonoudiou Pantelis
Department of Physiology, Anatomy and Genetics, University of Oxford||Oxford Ion Channel Initiative, University of OxfordDepartment of Neuroscience, Physiology and Pharmacology, University College LondonDepartment of Neuroscience, Physiology and Pharmacology, University College LondonDepartment of Neuroscience, Physiology and Pharmacology, University College LondonDepartment of Neuroscience, Physiology and Pharmacology, University College LondonDepartment of Neuroscience, Physiology and Pharmacology, University College LondonDepartment of Neuroscience, Physiology and Pharmacology, University College LondonDepartment of Neuroscience, Physiology and Pharmacology, University College LondonDepartment of Neuroscience, Physiology and Pharmacology, University College LondonDepartment of Physiology, Anatomy and Genetics, University of Oxford
基础医学神经病学、精神病学生理学
Mann Edward O.,Arancibia-Carcamo I. Lorena,Lopez-Domenech Guillermo,Salinas Patricia C.,Kittler Josef T.,Kontou Georgina,Higgs Nathalie F.,Podpolny Marina,Szulc Blanka R.,Antonoudiou Pantelis.Miro1-dependent Mitochondrial Dynamics in Parvalbumin Interneurons[EB/OL].(2025-03-28)[2025-05-09].https://www.biorxiv.org/content/10.1101/2020.10.06.328534.点此复制
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