Pericentrin is a Kinesin-1 Activator that Drives Centriole Motility
Pericentrin is a Kinesin-1 Activator that Drives Centriole Motility
Abstract Centrosome positioning is essential for their function. Typically, centrosomes are transported to various cellular locations through the interaction of centrosome nucleated microtubules with motor proteins. However, it remains unknown how centrioles migrate in cellular contexts in which centrioles do not nucleate microtubules. Here, we demonstrate that during interphase inactive centrioles move directly along the non-centrosomal microtubule network as cargo for the motor protein Kinesin-1. We identify Pericentrin-Like-Protein (PLP) as a novel Kinesin-1 interacting molecule essential for centriole motility. PLP directly interacts with the cargo binding domain of Kinesin-1 and they comigrate on microtubules in vitro. Finally, we demonstrate that PLP-Kinesin-1 dependent transport is essential for centrosome asymmetry age-dependent centrosome inheritance in asymmetric stem cell division.
Liu Rong、Combs Christian、Sellers James R.、Galletta Brian J.、Fagerstrom Carey J.、Hannaford Matthew R.、Rusan Nasser M.、Billington Neil、Swider Zachary T.
Cell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of HealthCell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of HealthCell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of HealthCell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of HealthCell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of HealthCell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of HealthCell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of HealthCell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of HealthCell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of Health
细胞生物学分子生物学
Liu Rong,Combs Christian,Sellers James R.,Galletta Brian J.,Fagerstrom Carey J.,Hannaford Matthew R.,Rusan Nasser M.,Billington Neil,Swider Zachary T..Pericentrin is a Kinesin-1 Activator that Drives Centriole Motility[EB/OL].(2025-03-28)[2025-07-01].https://www.biorxiv.org/content/10.1101/2022.01.12.476023.点此复制
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