Intragenic Complementation of Cellulose Synthase-Like D1 alleles in Root Hair Development
Intragenic Complementation of Cellulose Synthase-Like D1 alleles in Root Hair Development
Abstract Root hair cells form the primary interface of plants with the soil environment, playing key roles in nutrient uptake and plant defense. In addition, they are typically the first cells infected by nitrogen-fixing soil bacteria during the root nodule symbiosis. Here we report a role for the Cellulose Synthase-Like D1 (CSLD1) gene in root hair development in Lotus japonicus. CSLD1 belongs to the cellulose synthase protein family that includes cellulose synthases, and cellulose synthase-like proteins, the latter thought to be involved in the biosynthesis of hemicellulose. We describe 11 csld1 mutant alleles that have either short (Ljcsld1-1) or variable length roots hairs (Ljcsld1-2 to 11). Examination of Ljcsld1-1 and one variable-length root hair mutant, Ljcsld1-6 showed increased root hair cell wall thickness, which in Ljcsld1-1 was more pronounced, suggesting a possible link with the defect in root nodule symbiosis. In addition, Ljcsld1-1 heterozygotes had intermediate root hair lengths, between those of wild type and the homozygotes. Intragenic complementation was observed between alleles with mutations in the N-terminal domain and other alleles, suggesting modularity of CSLD1 function and that it may operate as a homodimer or multimer. One sentence summaryThis research describes novel gain- and loss-of-function mutations at the Lotus japonicus CELLULOSE SYNTHASE-LIKE D1 locus and analyzes their impact on root hair development.
Murray Jeremy Dale、Novero Mara、Inada Sayaka、Sakai Tatsuya、Bonfante Paola、Nakano Michiharu、Sato Sushei、Karas Bogumil J.、Ross Loretta、Szczyglowski Krzysztof、Amyot Lisa
Agriculture and Agri-Food Canada, London Research and Development Centre||National Key Laboratory of Plant Molecular Genetics, CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), CAS Center for Excellence in Molecular and Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesDepartment of Life Sciences and Systems Biology, University of TorinoRIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-kuGraduate School of Science and Technology, Niigata UniversityDepartment of Life Sciences and Systems Biology, University of TorinoRIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-kuGraduate School of Life Sciences, Tohoku UniversityDepartment of Biochemistry, The University of Western Ontario||Agriculture and Agri-Food Canada, London Research and Development CentreAgriculture and Agri-Food Canada, London Research and Development CentreAgriculture and Agri-Food Canada, London Research and Development Centre||Department of Biology, University of Western OntarioAgriculture and Agri-Food Canada, London Research and Development Centre
植物学遗传学细胞生物学
Murray Jeremy Dale,Novero Mara,Inada Sayaka,Sakai Tatsuya,Bonfante Paola,Nakano Michiharu,Sato Sushei,Karas Bogumil J.,Ross Loretta,Szczyglowski Krzysztof,Amyot Lisa.Intragenic Complementation of Cellulose Synthase-Like D1 alleles in Root Hair Development[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2020.03.02.973545.点此复制
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