Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging
Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging
Fluorescent biosensors revolutionized biomedical science by enabling the direct measurement of signaling activities in living cells, yet the current technology is limited in resolution and dimensionality. Here, we introduce highly sensitive chemigenetic kinase activity biosensors that combine the genetically encodable self-labeling protein tag HaloTag7 with bright far-red-emitting synthetic fluorophores. This technology enables five-color biosensor multiplexing, 4D activity imaging, and functional super-resolution imaging via stimulated emission depletion (STED) microscopy.
Wang Zichen、Hakozaki Hiroyuki、Li Yajuan、Lyons Anne C.、Rohm Theresa V.、Sch?neberg Johannes、Fraser Scott E.、Shi Lingyan、Mehta Sohum、Schneider Falk、Olefsky Jerrold M.、Frei Michelle S.、Zhang Jin、Liu Longwei、Wang Yingxiao、Sanchez Samantha A.
生物科学研究方法、生物科学研究技术生物化学分子生物学
Wang Zichen,Hakozaki Hiroyuki,Li Yajuan,Lyons Anne C.,Rohm Theresa V.,Sch?neberg Johannes,Fraser Scott E.,Shi Lingyan,Mehta Sohum,Schneider Falk,Olefsky Jerrold M.,Frei Michelle S.,Zhang Jin,Liu Longwei,Wang Yingxiao,Sanchez Samantha A..Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging[EB/OL].(2025-03-28)[2025-05-12].https://www.biorxiv.org/content/10.1101/2024.02.10.579766.点此复制
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