PD患者SIRT1/NF-κB信号通路与认知功能损害的相关性

Correlation between SIRT1/NF-κB signaling pathway and cognitive impairment in patients with Parkinsons diseaseZHANG Lixia, LANG Zegao, WANG Hui Department of Neurology, Taizhou Second people’s Hospital,Taizhou 317200,Zhejiang,ChinaAbstractObjective: To study the correlation and diagnostic significance of the silent information regulator 2 related enzyme 1 (SIRT1)/nuclear factor kappa B (NF-κB) signaling pathway and cognitive impairment (CI) in patients with Parkinsons disease. Method: 119 patients with primary PD diagnosed and treated be in Taizhou Second people’s Hospital from September 2017 to March 2021 were selected as the PD group. According to the cognitive function status of the research subjects and related diagnosis guidelines, the included research subjects were divided into cognitive functions There were 33 cases in the normal subgroup (PD-NC), 58 cases in the mild cognitive impairment subgroup (PD-MCI), and 28 cases in the dementia subgroup (PDD).In addition, 50 healthy people who came to be in the hospital for physical examination during the same period were selected as the normal control group. After admission, the peripheral blood levels of SIRT1/NF-κB signaling pathway related molecules in all subjects were detected, and the relationship between the expression of SIRT1/NF-κB signaling pathway and cognitive function in PD patients was analyzed by partial correlation analysis. PD patients were secondary The analysis of the influencing factors of CI adopts Cox proportional hazard regression model analysis, and adopts receiver operating characteristic (ROC) curve to evaluate the diagnostic efficacy of SIRT1/NF-κB signaling pathway related molecule expression in PD patients with secondary CI.Results: There was a statistically significant difference between the two groups in terms of education level, course of disease, HY staging, UPDRS-Ⅲ, MoCA total score and other baseline data among different subgroups of PD patients (P<0.05); SIRT1 in PD group mRNA expression was significantly lower than that of the normal group, while NF-κB mRNA expression was higher than that of the normal group (P<0.05). The PD group was further stratified and compared, and it was found that the SIRT1 mRNA expression of the PDD group was significantly lower than that of the PD-MCI group The expression of the above indicators in the PD-NC group and PD-MCI group was also significantly lower than that in the PD-NC group (P<0.05), while the expression of NF-κB mRNA showed the opposite trend; using partial correlation analysis, SIRT1 and NF-κB mRNA were found There is still a significant correlation with MoCA score (r=0.637, -0.527, P<0.05);The results of Cox regression analysis showed that high H-Y staging and high expression of NF-κB mRNA were independent risk factors for the occurrence of CI in PD patients, and high expression of SIRT1 mRNA and high education level were independent protective factors for PD patients. The ROC curve showed that SIRT1 NF-κB mRNA expression can diagnose secondary CI in PD patients (P<0.05), and the two are fitted to predict. At this time, the AUC, sensitivity, and specificity are 0.875, 86.81%, and 90.36%, respectively. Conclusion: SIRT1 and NF-κB mRNA are highly expressed in the peripheral blood of patients with PD secondary to CI, and have a strong correlation with this type of cognitive function. Early enhancement of the detection of SIRT1 and NF-κB mRNA expression in peripheral blood of PD patients can be Play an important role in diagnosis and prognosis.Keywords Parkinsons disease; SIRT1/NF-κB signaling pathway; cognitive impairment; correlation;