Characterisation of the SUF FeS cluster machinery in the amitochondriate eukaryote Monocercomonoides exilis
Characterisation of the SUF FeS cluster machinery in the amitochondriate eukaryote Monocercomonoides exilis
Abstract Monocercomonoides exilis is the first eukaryotic organism described as a complete amitochondriate, yet it shares common features with heterotrophic anaerobic/microaerophilic protists, some of which bear divergent mitochondrion-related organelles or MROs. It has been postulated that the retention of these organelles stems from their involvement in the assembly of essential cytosolic and nuclear FeS proteins, whose maturation requires the evolutionarily conserved mitochondrial ISC and cytosolic CIA machineries. The amitochondriate M. exilis lacks genes encoding the ISC machinery yet contains a bacteria-derived SUF system (MeSuf), composed of the cysteine desulphurase SufS fused to SufD and SufU, as well as the FeS scaffolding components MeSufB and MeSufC. Here, we show that expression of the M. exilis SUF genes, either individually or in tandem, can restore the maturation of the FeS protein IscR in the Escherichia coli double mutants of ΔsufS ΔiscS and ΔsufB ΔiscUA. In vivo and in vitro studies indicate that purified MeSufB, MeSufC and MeSufDSU proteins interact suggesting that they act as a complex in the protist. MeSufBC can undergo conformational changes in the presence of ATP and assemble FeS clusters under anaerobic conditions in presence and absence of ATP in vitro. Altogether, these results indicate that the dynamically interacting MeSufDSUBC proteins may function as an FeS cluster assembly complex in M. exilis thereby being capable of replacing the organelle-enclosed ISC system of canonical eukaryotes.
Pe?a-Diaz Priscila、Braymer Joseph J.、Zelen¨￠ Marie、Lill Roland、Hrdy Ivan、Hochberg Georg、Lometto Stefano、Treitli Sebastian C.、Py B¨|atrice、Vacek Vojt¨§ch、Hampl Vladim¨ar
Department of Parasitology, BIOCEV, Faculty of Science, Charles UniversityInstitut f¨1r Zytobiologie und Zytopathologie, Fachberich Medizin, Philipps-Universit?t Marburg||Zentrum f¨1r Synthetische Mikrobiologie Synmikro, Philipps-Universit?t MarburgDepartment of Parasitology, BIOCEV, Faculty of Science, Charles UniversityInstitut f¨1r Zytobiologie und Zytopathologie, Fachberich Medizin, Philipps-Universit?t Marburg||Zentrum f¨1r Synthetische Mikrobiologie Synmikro, Philipps-Universit?t MarburgDepartment of Parasitology, BIOCEV, Faculty of Science, Charles UniversityZentrum f¨1r Synthetische Mikrobiologie Synmikro, Philipps-Universit?t Marburg||Max Planck Institute for Terrestrial Microbiology||Department of Chemistry, University of MarburgMax Planck Institute for Terrestrial MicrobiologyDepartment of Parasitology, BIOCEV, Faculty of Science, Charles UniversityLaboratoire de Chimie Bact¨|rienne, CNRS-Aix Marseille Universit¨|Department of Parasitology, BIOCEV, Faculty of Science, Charles UniversityDepartment of Parasitology, BIOCEV, Faculty of Science, Charles University
分子生物学细胞生物学生物化学
Pe?a-Diaz Priscila,Braymer Joseph J.,Zelen¨￠ Marie,Lill Roland,Hrdy Ivan,Hochberg Georg,Lometto Stefano,Treitli Sebastian C.,Py B¨|atrice,Vacek Vojt¨§ch,Hampl Vladim¨ar.Characterisation of the SUF FeS cluster machinery in the amitochondriate eukaryote Monocercomonoides exilis[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2023.03.30.534840.点此复制
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