Flotillin mediated membrane fluidity controls peptidoglycan synthesis and MreB movement
Flotillin mediated membrane fluidity controls peptidoglycan synthesis and MreB movement
Abstract The bacterial plasma membrane is an important cellular compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest that flotillin proteins are required for the formation of complexes of membrane proteins including cell-wall synthetic proteins. We show here that bacterial flotillins are important factors for membrane fluidity homeostasis. Loss of flotillins leads to a decrease in membrane fluidity that in turn leads to alterations in MreB dynamics and, as a consequence, in peptidoglycan synthesis. These alterations are reverted when membrane fluidity is restored by a chemical fluidizer. In vitro, the addition of a flotillin increases membrane fluidity of liposomes. Our data support a model in which flotillins are required for direct control of membrane fluidity rather than for the formation of protein complexes via direct protein-protein interactions.
Scheffers Dirk-Jan、Hartman Alwin M.、Hirsch Anna K. H.、Zieli¨?ska Aleksandra、Savietto Abigail、de Sousa Borges Anabela、Berbon Melanie、de Boer Rinse、Habenstein Birgit、Bramkamp Marc、Roelofsen Jo?l R.、van der Klei Ida J.、Martinez Denis
Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenDepartment of Drug Design and Optimization (DDOP), Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI)||Department of Pharmacy, Saarland University||Stratingh Institute for Chemistry, University of GroningenDepartment of Drug Design and Optimization (DDOP), Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI)||Department of Pharmacy, Saarland University||Stratingh Institute for Chemistry, University of GroningenMolecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenBiozentrum, Ludwig-Maximilians-Universit?t M¨1nchen||Institute for General Microbiology, Christian-Albrechts-University KielMolecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenInstitute of Chemistry & Biology of Membranes & Nanoobjects (UMR5248 CBMN), IECB, CNRS, Universit¨| Bordeaux, Institut Polytechnique BordeauxMolecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenInstitute of Chemistry & Biology of Membranes & Nanoobjects (UMR5248 CBMN), IECB, CNRS, Universit¨| Bordeaux, Institut Polytechnique BordeauxBiozentrum, Ludwig-Maximilians-Universit?t M¨1nchen||Institute for General Microbiology, Christian-Albrechts-University KielMolecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenStratingh Institute for Chemistry, University of GroningenInstitute of Chemistry & Biology of Membranes & Nanoobjects (UMR5248 CBMN), IECB, CNRS, Universit¨| Bordeaux, Institut Polytechnique Bordeaux
细胞生物学分子生物学微生物学
Scheffers Dirk-Jan,Hartman Alwin M.,Hirsch Anna K. H.,Zieli¨?ska Aleksandra,Savietto Abigail,de Sousa Borges Anabela,Berbon Melanie,de Boer Rinse,Habenstein Birgit,Bramkamp Marc,Roelofsen Jo?l R.,van der Klei Ida J.,Martinez Denis.Flotillin mediated membrane fluidity controls peptidoglycan synthesis and MreB movement[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/736819.点此复制
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