NIR-II fluorescence microscopic imaging of cortical vasculature in non-human primates
NIR-II fluorescence microscopic imaging of cortical vasculature in non-human primates
Abstract Vasculature architecture in the brain can provide revealing information about mental and neurological function and disease. Fluorescence imaging in the second near-infrared (NIR-II) regime with less light scattering is a more promising method for detecting cortical vessels than traditional visible and NIR-I modes. Here, for the first time, we developed, NIR-II fluorescence microscopy capabilities for imaging brain vasculature in macaque monkey. The first is a wide-field microscope with high temporal resolution (25 frames/second) for measuring blood flow velocity and cardiac impulse period, and the second is a high spatial resolution (<10 μm) confocal microscope producing three-dimensional maps of the cortical microvascular network (～500 μm deep). Both were designed with flexibility to image various cortical locations on the head. Use of a clinically approved dye provided high brightness in NIR-II region. This comprises an important advance towards studies of neurovascular coupling, stroke, and other diseases relevant to neurovascular health in humans.
Wang Mengqi、Qian Jun、Xi Wang、Roe Anna Wang、Zhu Liang、Cai Zhaochong
Interdisciplinary Institute of Neuroscience and Technology (ZIINT), College of Biomedical Engineering and Instrument Science, Zhejiang UniversityState Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, Zhejiang UniversityInterdisciplinary Institute of Neuroscience and Technology (ZIINT), the Second Affiliated Hospital, School of Medicine, Zhejiang University||Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang UniversityInterdisciplinary Institute of Neuroscience and Technology (ZIINT), the Second Affiliated Hospital, School of Medicine, Zhejiang University||Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang UniversityInterdisciplinary Institute of Neuroscience and Technology (ZIINT), College of Biomedical Engineering and Instrument Science, Zhejiang UniversityState Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, Zhejiang University
医学研究方法基础医学神经病学、精神病学
Wang Mengqi,Qian Jun,Xi Wang,Roe Anna Wang,Zhu Liang,Cai Zhaochong.NIR-II fluorescence microscopic imaging of cortical vasculature in non-human primates[EB/OL].(2025-03-28)[2025-06-07].https://www.biorxiv.org/content/10.1101/853200.点此复制
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