Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
Summary Synaptic diversity is a key feature of neural circuits. The structural and functional diversity of closely spaced inputs converging on the same neuron suggests that cell-surface interactions are essential in organizing input properties. Here, we analyzed the cell-surface protein (CSP) composition of hippocampal mossy fiber (MF) inputs on CA3 pyramidal neurons to identify regulators of MF-CA3 synapse properties. We uncover a rich cell-surface repertoire that includes adhesion proteins, guidance cue receptors, extracellular matrix (ECM) proteins, and uncharacterized CSPs. Interactome screening reveals multiple ligand-receptor modules and identifies ECM protein Tenascin-R (TenR) as a ligand of the uncharacterized neuronal receptor IgSF8. Presynaptic Igsf8 deletion impairs MF-CA3 synaptic architecture and robustly decreases the density of bouton filopodia that provide feedforward inhibition of CA3 neurons. Consequently, loss of IgSF8 increases CA3 neuron excitability. Our findings identify IgSF8 as a regulator of CA3 microcircuit development and suggest that combinations of CSP modules define input identity.
Smukowski Samuel N.、Vanderlinden Jeroen、Rybakin Vasily、ten Bos Jolijn、Vennekens Kristel M.、Wierda Keimpe D.、Portegies Sybren、de Wit Joris、Ap¨?stolo Nuno、Condomitti Giuseppe、Comoletti Davide、Savas Jeffrey N.、Gounko Natalia V.、Trobiani Laura
Department of Neurology, Northwestern University Feinberg School of MedicineVIB Center for Brain & Disease Research||KU Leuven, Department of Neurosciences, Leuven Brain InstituteImmunobiology, REGA Institute, Department of Microbiology and ImmunologyVIB Center for Brain & Disease Research||KU Leuven, Department of Neurosciences, Leuven Brain InstituteVIB Center for Brain & Disease Research||KU Leuven, Department of Neurosciences, Leuven Brain InstituteVIB Center for Brain & Disease Research||KU Leuven, Department of Neurosciences, Leuven Brain InstituteVIB Center for Brain & Disease Research||KU Leuven, Department of Neurosciences, Leuven Brain InstituteVIB Center for Brain & Disease Research||KU Leuven, Department of Neurosciences, Leuven Brain InstituteVIB Center for Brain & Disease Research||KU Leuven, Department of Neurosciences, Leuven Brain InstituteVIB Center for Brain & Disease Research||KU Leuven, Department of Neurosciences, Leuven Brain InstituteSchool of Biological Sciences, Victoria University of Wellington||Child Health Institute of New Jersey, and Departments of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Rutgers UniversityDepartment of Neurology, Northwestern University Feinberg School of MedicineVIB Center for Brain & Disease Research||KU Leuven, Department of Neurosciences, Leuven Brain Institute||Electron Microscopy Platform & VIB BioImaging CoreSchool of Biological Sciences, Victoria University of Wellington
细胞生物学分子生物学生理学
Smukowski Samuel N.,Vanderlinden Jeroen,Rybakin Vasily,ten Bos Jolijn,Vennekens Kristel M.,Wierda Keimpe D.,Portegies Sybren,de Wit Joris,Ap¨?stolo Nuno,Condomitti Giuseppe,Comoletti Davide,Savas Jeffrey N.,Gounko Natalia V.,Trobiani Laura.Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer[EB/OL].(2025-03-28)[2025-04-30].https://www.biorxiv.org/content/10.1101/846816.点此复制
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