Immunogenicity and pre-clinical efficacy of an OMV-based SARS-CoV-2 vaccine
Immunogenicity and pre-clinical efficacy of an OMV-based SARS-CoV-2 vaccine
Abstract The vaccination campaign against SARS-CoV-2 relies on the world-wide availability of effective vaccines, with a potential need of 20 billion vaccine doses to fully vaccinate the world population. To reach this goal, the manufacturing and logistic processes should be affordable to all countries, irrespectively of economical and climatic conditions. Outer membrane vesicles (OMVs) are bacterial-derived vesicles that can be engineered to incorporate heterologous antigens. Given the inherent adjuvanticity, such modified OMVs can be used as vaccine to induce potent immune responses against the associated protein. Here we show that OMVs engineered to incorporate peptides derived from the receptor binding motif (RBM) of the spike protein from SARS-CoV-2 elicit an effective immune response in immunized mice, resulting in the production of neutralizing antibodies. The immunity induced by the vaccine is sufficient to protect K18-hACE2 transgenic mice from intranasal challenge with SARS-CoV-2, preventing both virus replication in the lungs and the pathology associated with virus infection. Furthermore, we show that OMVs can be effectively decorated with RBM peptides derived from a different genetic variant of SARS-CoV-2, inducing a similarly potent neutralization activity in vaccinated mice. Altogether, given the convenience associated with ease of engineering, production and distribution, our results demonstrate that OMV-based SARS-CoV-2 vaccines can be a crucial addition to the vaccines currently available.
Di Lucia Pietro、Iannacone Matteo、Cherepanov Peter、Bolognesi Martino、Grandi Guido、Di Lascio Gabriele、Piubelli Chiara、Zipeto Donato、Rav¨¤ Micol、Fumagalli Valeria、Carbonare Luca Dalle、Sala Eleonora、Vanzo Teresa、Grandi Alberto、Caproni Elena、Bertelli Cinzia、Gagliardi Assunta、Tomasi Michele、Tamburini Silvia、Marotta Davide、Fantappi¨¨ Laura、Valenti Maria Teresa、Bisoffi Zeno、Pizzato Massimo
IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious DiseasesIRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Diseases||Vita-Salute San Raffaele University||IRCCS San Raffaele Scientific Institute, Experimental Imaging CenterThe Francis Crick Institute, Chromatin Structure and Mobile DNA LaboratoryUniversity of MilanUniversity of Trento, CIBIO DepartmentUniversity of Trento, CIBIO Department||Toscana Life Sciences FoundationIRCCS Sacro Cuore Don Calabria Hospital, Department of Infectious, Tropical Diseases and MicrobiologyUniversity of Verona, Department of Neuroscience, Biomedicine and Movement SciencesIRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious DiseasesIRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Diseases||Vita-Salute San Raffaele UniversityUniversity of Verona, Department of Medicine, Biomedicine and Movement SciencesIRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Diseases||Vita-Salute San Raffaele UniversityUniversity of Trento, CIBIO DepartmentToscana Life Sciences Foundation||BiOMViS SrlUniversity of Trento, CIBIO Department||Toscana Life Sciences FoundationUniversity of Trento, CIBIO DepartmentToscana Life Sciences FoundationUniversity of Trento, CIBIO DepartmentUniversity of Trento, CIBIO Department||Toscana Life Sciences FoundationIRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Diseases||Vita-Salute San Raffaele UniversityToscana Life Sciences FoundationUniversity of Verona, Department of Medicine, Biomedicine and Movement SciencesIRCCS Sacro Cuore Don Calabria Hospital, Department of Infectious, Tropical Diseases and MicrobiologyUniversity of Trento, CIBIO Department
预防医学基础医学生物科学研究方法、生物科学研究技术
Di Lucia Pietro,Iannacone Matteo,Cherepanov Peter,Bolognesi Martino,Grandi Guido,Di Lascio Gabriele,Piubelli Chiara,Zipeto Donato,Rav¨¤ Micol,Fumagalli Valeria,Carbonare Luca Dalle,Sala Eleonora,Vanzo Teresa,Grandi Alberto,Caproni Elena,Bertelli Cinzia,Gagliardi Assunta,Tomasi Michele,Tamburini Silvia,Marotta Davide,Fantappi¨¨ Laura,Valenti Maria Teresa,Bisoffi Zeno,Pizzato Massimo.Immunogenicity and pre-clinical efficacy of an OMV-based SARS-CoV-2 vaccine[EB/OL].(2025-03-28)[2025-06-27].https://www.biorxiv.org/content/10.1101/2021.07.12.452027.点此复制
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