Reaction-diffusion patterning of DNA-based artificial cells
Reaction-diffusion patterning of DNA-based artificial cells
Abstract Biological cells display complex internal architectures, with distinct micro environments that establish the chemical heterogeneity needed to sustain cellular functions. The continued efforts to create advanced cell mimics – artificial cells – demands strategies to construct similarly heterogeneous structures with localized functionalities. Here, we introduce a platform for constructing membrane-less artificial cells from the self-assembly of synthetic DNA nanostructures, in which internal domains can be established thanks to prescribed reaction-diffusion waves. The method, rationalized through numerical modeling, enables the formation of up to five distinct, concentric environments, in which functional moieties can be localized. As a proof-of-concept, we apply this platform to build DNA-based artificial cells in which a prototypical nucleus synthesizes fluorescent RNA aptamers, which then accumulate in a surrounding storage shell, thus demonstrating spatial segregation of functionalities reminiscent of that observed in biological cells.
Kotar Jurij、Cicuta Pietro、Brady Ryan A.、Booth Michael J.、Di Michele Lorenzo、Al Samad Assala、Walczak Michal、Leathers Adrian
Biological and Soft Systems, Cavendish Laboratory, University of CambridgeBiological and Soft Systems, Cavendish Laboratory, University of CambridgeDepartment of Chemistry, Faculty of Natural and Mathematical Sciences, King?ˉs College LondonChemistry Research Laboratory, University of Oxford||Department of Chemistry, University College LondonDepartment of Chemistry, Imperial College London, Molecular Sciences Research Hub||fabriCELL, Imperial College London, Molecular Sciences Research Hub||Biological and Soft Systems, Cavendish Laboratory, University of CambridgeChemistry Research Laboratory, University of Oxford||Department of Chemistry, University College LondonBiological and Soft Systems, Cavendish Laboratory, University of CambridgeBiological and Soft Systems, Cavendish Laboratory, University of Cambridge
细胞生物学生物工程学分子生物学
Kotar Jurij,Cicuta Pietro,Brady Ryan A.,Booth Michael J.,Di Michele Lorenzo,Al Samad Assala,Walczak Michal,Leathers Adrian.Reaction-diffusion patterning of DNA-based artificial cells[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2022.03.24.485404.点此复制
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