Evidence for loss and adaptive reacquisition of alcoholic fermentation in an early-derived fructophilic yeast lineage
Evidence for loss and adaptive reacquisition of alcoholic fermentation in an early-derived fructophilic yeast lineage
Abstract Fructophily is a rare trait that consists in the preference for fructose over other carbon sources. Here we show that in a yeast lineage (the Wickerhamiella/Starmerella, W/S clade) formed by fructophilic species thriving in the floral niche, the acquisition of fructophily is part of a wider process of adaptation of central carbon metabolism to the high sugar environment. Coupling comparative genomics with biochemical and genetic approaches, we show that the alcoholic fermentation pathway was profoundly remodeled in the W/S clade, as genes required for alcoholic fermentation were lost and subsequently re-acquired from bacteria through horizontal gene transfer. We further show that the reinstated fermentative pathway is functional and that an enzyme required for sucrose assimilation is also of bacterial origin, reinforcing the adaptive nature of the genetic novelties identified in the W/S clade. This work shows how even central carbon metabolism can be remodeled by a surge of HGT events.
Rokas Antonis、Shen Xing-Xing、Leandro Maria Jos¨|、Gon?alves Carla、Wisecaver Jennifer H.、Salema-Oom Madalena、Peris David、Hittinger Chris Todd、Gon?alves Paula
Department of Biological Sciences, Vanderbilt UniversityDepartment of Biological Sciences, Vanderbilt UniversityInstituto de Tecnologia Qu¨amica e Biol¨?gica Ant¨?nio Xavier, Universidade Nova de Lisboa||LNEG ¨C Laborat¨?rio Nacional de Energia e GeologiaUCIBIO-REQUIMTE, Departamento de Ci¨oncias da Vida, Faculdade de Ci¨oncias e Tecnologia, Universidade Nova de LisboaDepartment of Biological Sciences, Vanderbilt University||Department of Biochemistry, Purdue UniversityUCIBIO-REQUIMTE, Departamento de Ci¨oncias da Vida, Faculdade de Ci¨oncias e Tecnologia, Universidade Nova de Lisboa||Centro de Investiga??o Interdisciplinar Egas Moniz, Instituto Superior Ci¨oncias da Sa¨2de Egas MonizLaboratory of Genetics, Genome Center of Wisconsin, DOE Great Lakes Bioenergy Research Center, J. F. Crow Institute for the Study of Evolution, Wisconsin Energy Institute, University of Wisconsin-Madison||Department of Food Biotechnology, Institute of Agrochemistry and Food Technology (IATA), CSICLaboratory of Genetics, Genome Center of Wisconsin, DOE Great Lakes Bioenergy Research Center, J. F. Crow Institute for the Study of Evolution, Wisconsin Energy Institute, University of Wisconsin-MadisonUCIBIO-REQUIMTE, Departamento de Ci¨oncias da Vida, Faculdade de Ci¨oncias e Tecnologia, Universidade Nova de Lisboa
分子生物学微生物学生物化学
Rokas Antonis,Shen Xing-Xing,Leandro Maria Jos¨|,Gon?alves Carla,Wisecaver Jennifer H.,Salema-Oom Madalena,Peris David,Hittinger Chris Todd,Gon?alves Paula.Evidence for loss and adaptive reacquisition of alcoholic fermentation in an early-derived fructophilic yeast lineage[EB/OL].(2025-03-28)[2025-06-24].https://www.biorxiv.org/content/10.1101/213686.点此复制
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