首页|Na?ve and in vitro -activated primary mouse CD8 + T cells retain in vivo immune responsiveness after electroporation-based CRISPR/Cas9 genetic engineering
Na?ve and in vitro -activated primary mouse CD8 + T cells retain in vivo immune responsiveness after electroporation-based CRISPR/Cas9 genetic engineering
Na?ve and in vitro -activated primary mouse CD8 + T cells retain in vivo immune responsiveness after electroporation-based CRISPR/Cas9 genetic engineering
生物科学研究方法、生物科学研究技术基础医学分子生物学
Yerly Laura,Abe Jun,Pfenninger Petra.Na?ve and in vitro -activated primary mouse CD8 + T cells retain in vivo immune responsiveness after electroporation-based CRISPR/Cas9 genetic engineering[EB/OL].(2025-03-28)[2025-09-24].https://www.biorxiv.org/content/10.1101/2021.09.14.460345.点此复制
Abstract
CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells. Although its use is gaining momentum in studies on CD8+ T cell biology, it remains elusive to what extent CRISPR/Cas9 affects in vivo function of CD8+ T cells. Here, we optimized nucleofection-based CRISPR/Cas9 genetic engineering of na?ve and in vitro-activated primary mouse CD8+ T cells and tested their in vivo immune responses. Nucleofection of na?ve CD8+ T cells preserved their in vivo antiviral immune responsiveness to an extent that is indistinguishable from non-nucleofected cells, whereas in vitro activation of CD8+ T cells prior to nucleofection led to slightly impaired expansion/survival. Of note, different target proteins displayed distinct decay rates after gene editing. This is in stark contrast to a comparable period of time required to complete gene inactivation. Thus, for optimal experimental design, it is crucial to determine the kinetics of the loss of target gene product to adapt incubation period after gene editing. In sum, nucleofection-based CRISPR/Cas9 genome editing achieves efficient and rapid generation of mutant CD8+ T cells without imposing detrimental constraints on their in vivo functions.
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